McCoy cell cultures were inoculated with 121 urethral and cervical specimens taken from patients attending one of two sexually transmitted disease clinics. The mean number of Chlamydia trachomatis inclusions was greater when the cultures were inoculated with the specimens and centrifuged in the clinic than when the specimens were first stored in liquid nitrogen. Furthermore, 18 of the 29 chlamydia-positive specimens produced larger numbers of of inclusions when inoculated immediately. Despite this, the isolation rate from specimens inoculated directly (22%) was about the same as from specimens which had been frozen (21%). Of the 30 occasions on which the cell monolayers were disrupted, 29 followed immediate inoculation. This may possibly have been due to some toxic factor in some of the specimens.
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