An immuno-peroxidase technique using monoclonal antibodies against the major capsid protein L1 of HPV-16 was compared with dot-blot hybridisation of cervical scrapes and in situ hybridisation of cervical biopsy specimens for HPV-16 DNA. In a series of 20 patients all techniques were specific for HPV-16 infection. Ten patients were positive by dot-blot hybridisation and half of those were positive by in situ hybridisation. Only one of HPV-16 DNA positive cases showed L1 protein expression, apparently shortly after the onset of clinical infection. Whether major capsid protein expression reflects episodes of viral replication deserves further study.
Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.