Article Text

PDF

Use of the polymerase chain reaction for the detection of Chlamydia trachomatis in clinical specimens and its comparison to commercially available tests.
  1. J L Martin,
  2. S Y Alexander,
  3. T S Selwood,
  4. G F Cross
  1. Department of Microbiology, Monash University Medical School, Prahran, Victoria, Australia.

    Abstract

    A polymerase chain reaction (PCR) was developed to detect Chlamydia trachomatis in genital tract specimens. Two sets of primers for the PCR were used; one set amplifies a region of the plasmid present in all C trachomatis strains and the other amplifies a conserved region of the genome coding for the major outer membrane protein. The sensitivity of these PCRs were compared with each other, and with the sensitivities of antigen ELISA, Clearview and culture. Southern blotting and probing was used to increase sensitivity of detection.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.