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Pooling urine samples for PCR screening of C trachomatis urogenital infection in women
  1. J P Gomes1,
  2. M A Ferreira1,
  3. A Brito de Sá2,
  4. M A Catry3
  1. 1Bacteriology Department, National Institute of Health, Lisbon, Portugal
  2. 2Institute of Preventive Medicine, Lisbon Medical School, Lisbon, Portugal
  3. 3Bacteriology Department, National Institute of Health, Lisbon, Portugal
  1. João Paulo Gomes, Centro de Bacteriologia, Instituto Nacional de Saúde, Av Padre Cruz, 1649–016, Lisboa, Portugal

https://doi.org/10.1136/sti.77.1.76-a

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    Editor,—Selective or universal screening for Chlamydia trachomatis infections has been sugested by the World Health Organization as a primary prevention strategy.1

    The improved sensitivity of the nucleic acid amplification assays for the detection of C trachomatis allows the use of urine samples, suitable for screening programmes. However, these commercial assays are expensive, which make them disadvantageous for this purpose.

    Therefore, some authors have recently evaluated the accuracy and cost saving of different urine pooling strategies using polymerase chain reaction (PCR) and ligase chain reaction (LCR) tests for the screening for genital C trachomatis infections, reporting very encouraging results.2–5 As the pooling strategies need individual …

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