Table 1
Peripartum HIV test results
| Time (in weeks of gestation) | ||||
|---|---|---|---|---|
| 1 T = 12 weeks (“Booking blood”) | 2 T = 29 weeks | 3 T = 33 weeks (“Booking blood”) | 4 T = 13 weeks post partum (child presents) | |
| αEnzyme immunoassay (EIA) for detection of antibody to HIV-1 and 2. Biochem Immunosystems Inc, Montreal, Quebec, Canada. | ||||
| βEIA for detection of antibody to HIV-1 (Abbott Murex) Murex Biotech Ltd, Dartford, UK. | ||||
| γMicroparticle EIA for qualitative detection of antibodies to HIV-1 and 2. Abbott Laboratories, IL, USA. | ||||
| δEIA for detection of antibodies to HIV-1 and 2 (Abbott Murex) Murex Biotech Ltd, Dartford, UK. | ||||
| εPassive particle agglutination test for detection of antibodies to HIV-1 and 2 Fujirebio Inc, Tokyo, Japan. | ||||
| ζWestern blot for detection of antibodies to HIV antigens. Genelabs Diagnostics, Singapore. | ||||
| ηPolymerase chain reaction (PCR) for quantitative detection of HIV-1 RNA. Roche Diagnostics, Branchburg, NJ, USA. | ||||
| θSignal amplification nucleic acid probe assay for quantitative detection of HIV-1 RNA. Chiron Corp Emeryville, CA, USA. | ||||
| Hospital where blood taken | X | Y | Y | St Mary's |
| Blood was stored and retrospectively tested | Index antenatal test (serum not available for repeat retrospective testing) | Blood was stored and retrospectively tested | Postnatal test. Blood stored | |
| HIV antibody screening tests | Clear negative | Clear negative | Weak positive | Strong positive |
| i Detect-HIVα | i Abbot Axsym HIV 1/2 gOγ | i Murex HIV 1+2δ | i Abbot Axsym HIV 1/2 gOγ | |
| OD=−0.030, CO=0.144 | S/CO=0.42 | OD=0.938, CO=0.252 | OD=14.86, CO=1.00 | |
| ii Wellcozyme HIV | ii Wellcozyme HIV | ii Detect-HIVα | ||
| Recombinantβ | Recombinantβ | |||
| OD=1.179, CO=0.696 | OD=0.486, CO=0.839 | OD=2.050, CO=0.152 | ||
| iii Serodia HIV-1/2ε | iii Wellcozyme HIV Recombinantβ | |||
| HIV 1:1/256, HIV 2: <1/32 | OD=0.062, CO=0.532 | |||
| HIV specific antibody tests (CPHL in-house EIAs) | Clear negatives, (OD/CO) HIV | — | Strong positives, (OD/CO) HIV | Strong positives for IgG and IgA; weak positive IgM (OD/CO) HIV IgG=15.41, IgM=3.14, IgA=4.18. *Note decreasing values for IgM and IgA compared to previous |
| IgG=0.49, IgM=0.36, IgA=0.44 | IgG=12.34, IgM=10.94, IgA=5.28 | |||
| HIV western blotζ | — | — | — | HIV1 gag p17+, p24+++, p55+; pol p31++, p51++, p66+++; env gp41−, gp120+, gp160+++ |
| HIV2 gp36− | ||||
| HIV RNA (copies/ml) | Not detected | — | — | 41377 Quantiplex HIV-1 RNA 3.0θ |
| (< Limit of detection) Cobas Amplicor HIV-1 Monitor v1.5η | 82400 Cobas Amplicor HIV-1 Monitor v1.5η | |||
