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Prevalence of Chlamydia trachomatis infections in women attending six women's healthcare units in Kaunas, Lithuania
  1. M Domeika1,
  2. R Butylkina2,
  3. A Hallén3,
  4. T Spukaite4,
  5. V Juceviciute4,
  6. D Morkunaite5,
  7. R Jakutiene6,
  8. V Paliuniene7,
  9. J Barakauskiene8,
  10. M Goberis9
  1. 1Unit of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
  2. 2Kaunas Public Health Center, Lithuania
  3. 3Unit Dermato-venereology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden
  4. 4Kaunas Public Health Center, Lithuania
  5. 5Centro, Gynecologic Outpatient Clinic, Kaunas, Lithuania
  6. 6Shilainiu, Gynecologic Outpatient Clinic, Kaunas, Lithuania
  7. 7II Hospital's Gynecologic Outpatient Clinic, Kaunas, Lithuania
  8. 8Shilainiu, Gynecologic Outpatient Clinic, Kaunas, Lithuania
  9. 9Mazylio Antenatal Clinic, Kaunas, Lithuania
  1. Marius Domeika, PhD, Department of Medical Sciences, Unit of Clinical Bacteriology, Box 552, 751 22 Uppsala, Sweden Marius.Domeika{at}medsci.uu.se

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Introduction

Knowledge about the morbidity caused by Chlamydia trachomatis in eastern Europe is still insufficient. Reporting systems of sexually transmitted diseases and diagnostic tools, especially for the diagnosis of chlamydial infections, are still suboptimal,1 epidemiological studies are costly, and national resources devoted to STD prevention and control are small.

The aim of this study was to investigate the prevalence of C trachomatis infections in Lithuanian women, attending six main healthcare units in Kaunas, the second biggest Lithuanian town (500 000 inhabitants) and to learn about risk factors related to genital chlamydial infections.

Materials and methods

Women (n=1008) attending four gynaecological outpatient clinics and two antenatal clinics in Kaunas (Lithuania) between November 1999 and December 2000 were enrolled.

Study participants were given a standardised questionnaire concerning social status, sexual behaviour and contraceptive habits, medical and sexual history, and presence of genitourinary symptoms. Pelvic examination was carried out using a standardised examination protocol.

Direct microscopy of the vaginal wet mounts, methylene blue stained urethral and cervical smears was done “bedside.” The direct immunofluorescence (DIF) test (Syva MicroTrak Chlamydia trachomatis Direct Specimen Test, Trinity biotech, Ireland) was used for chlamydia antigen detection.

Results

The median age of the population tested was 25 (mean age 26.1) years. Of the patients who answered the question about the reason of visiting, 513 (59.2%) came for symptom evaluation, 300 (34.6%) for a regular check up, and 53 (6.1%) for a test of cure. There was no significant difference in the reason to attend different clinics.

The overall prevalence of C trachomatis infection was 8.4%. The highest prevalence of C trachomatis was observed in women below 19 years of age (17.4%), in women 20–40 years decreasing to 6.1–7.9%. In women older than 40 years, there was seen to be a further decrease to 2.9%.

There was a significant difference between the medical facility and the prevalence of C trachomatis infections. Thus, C trachomatis positive patients were: 6.7% of the women consulting OPGC I; 4.5%, OPGC II; 4.0%, OPGC III; 11.3%, OPGC IV; 9.5%, AC I; and 13.5%, AC II, respectively (p<0.001).

Figure 1 demonstrates the association between the percentage of young people (below 19 years of age) attending a certain health facility and the prevalence of C trachomatis infection.

Figure 1

Prevalence of C trachomatis infections and percentage of women aged below 19 years in 1008 women attending six women's health units in Kaunas, Lithuania.

Trichomonas vaginalis was detected in the wet mounts of 2.9%, candida in 14.3%, and bacterial vaginosis in 14.1% of the women tested. Neisseria gonorrhoeae was detected in 0.4% of the cervical smears.

Smokers (n = 243; 24.3%) were significantly more often chlamydia positive compared with non-smokers (13.2% v 7%, OR 2.0, 95%CI 1.3–3.2; p<0.005). Smokers had significantly often more than two sexual partners during the last 2 months compared with non-smokers (41.3% v 23.6%, OR 2.3, 95%CI 1.2–4.2). Significantly more smokers had had their first sexual intercourse at below 18 years of age (p<0.001).

The median age at sexual debut was 18 years (mean 19 (SD 2.7)). The median number of partners during the last 6 months was 1 (range 1–2), during 12 months 1 (1–7), during their lifetime, 1 (1–50). Significantly more women who started their sexual life before 18 and had more than one sexual partner during the last 6 or 12 months had a chlamydial infection.

The reason for attending, marital status, education, occupation, past or present genitourinary symptoms, a history of reproductive tract infections, day of the menstrual cycle, child birth, legal abortions or miscarriages did not affect the incidence of C trachomatis infection.

Approximately one fourth of the women could not answer the question about their sexual partner's genital symptoms, if any, or if he was tested for any reproductive tract infection, neither did they know if he had ever had any infection.

C trachomatis positive women more often had cervical discharge (44% v 22%, OR 2.7, 95% CI 1.7–44.; p<0.000), which was mostly mucopurulent (37% v 10%, OR 3.0, 95% CI 1.3–6.1, p<0.000).

C trachomatis positive women significantly more often had concomitant infections with T vaginalis (7.1% v 2.5%, OR 2.9, 95%CI 1.1–7.1, p =0.02) and N gonorrhoeae (2.4% v 0.3%, OR 7.3, 95%CI 1.0–45.1, p=0.02), as well as bacterial vaginosis (21.2% v 13.5%, OR 1.7, 95%CI 1.0–2.9, p=0.05) and cervicitis (52.9% v 10.5%, R 9.6, 95% CI 6.0–15.5, p=0.00). There was no significant difference in the number of candida infections or the finding of urethritis between C trachomatis positive and negative women.

Discussion

In the present study we found the prevalence of C trachomatis infection to vary between six different healthcare units from 4% to 13.5%. This difference was not caused by differences in reasons for visiting but by the proportion of visitors below 19 years of age. This group had a prevalence five to eight times greater than that of the following 5 years age groups. In a previous study conducted on the female population in Klaipeda,2 the prevalence peak was at 24 years of age. This exemplifies that when tailoring prevention programmes one has to be aware of the age specific prevalence.

In this study smoking was associated with chlamydial infection. Probably smoking itself was not a risk factor, but smoking women significantly more often had had more than two sexual partners during the last 6 and 12 months. They also became sexually active earlier—that is, smokers belong to a group with “risky behaviour,” a fact also noted by others.3

The presence of current symptoms or a history of reproductive tract infections did not influence the presence or absence of C trachomatis infections in the present study. This could reflect the women's awareness of symptomatology in general.

Cervical discharge, especially of a mucopurulent character,4,5 is a well known marker for having genital chlamydial infection. These signs were significantly more often expressed in C trachomatis positive women in this study.

Since most STDs have common risk factors, anyone with one infection diagnosed is at higher risk of having several infections.6 This is also supported by the present study.

Acknowledgments

Funding: Swedish Institute of Infectious Disease Control.

Conflict of interest: none.

Contributors: MD initiated, designed and supervised the study, worked on this paper, and conducted statistical analysis; AH co-supervised the study and was working on the manuscript; RB was helping with the study design, its technical performance, data computing and interpretation; TS and VJ were helping with the study design, its laboratory performance, and interpretation; DM, RJ, VP, JB, and MG were responsible for sample collection and evaluation at the study site.

References

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