Article Text


Trichomoniasis: clinical manifestations, diagnosis and management
  1. H Swygard,
  2. A C Seña,
  3. M M Hobbs,
  4. M S Cohen
  1. The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
  1. Correspondence to:
 Heidi Swygard
 CB # 7030, 130 Mason Farm Road, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7030, USA;


Trichomonas vaginalis was originally considered a commensal organism until the 1950s when the understanding of its role as a sexually transmitted infection (STI) began to evolve. Trichomoniasis has been associated with vaginitis, cervicitis, urethritis, pelvic inflammatory disease (PID), and adverse birth outcomes. Infection with T vaginalis could have an important role in transmission and acquisition of HIV. T vaginalis is site specific for the genitourinary tract and has been isolated from virtually all genitourinary structures. Asymptomatic disease is common in both men and women, thus screening for disease is important. Various sociodemographic factors have been correlated with presence of T vaginalis, and may be used to predict infection. Diagnosis is usually made from wet mount microscopy and direct visualisation, which are insensitive. DNA amplification techniques perform with good sensitivity, but are not yet approved for diagnostic purposes. In areas where diagnostic methods are limited, management of trichomoniasis is usually as part of a clinical syndrome; vaginal discharge for women and urethral discharge for men. A single dose of metronidazole is effective in the majority of cases. Outside of the United States, other nitroimidazoles may be used and are as effective as metronidazole. Metronidazole resistance is an emerging problem, but its clinical importance is not yet clear. Concomitant treatment of sexual partners is recommended.

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Trichomoniasis is a sexually transmitted disease (STI) with important public health ramifications; it has been associated with vaginitis, cervicitis, urethritis, and pelvic inflammatory disease (PID). Trichomoniasis also impacts upon birth outcomes and is a co-factor in human immunodeficiency virus (HIV) transmission and acquisition.1

Approximately 180 million women worldwide may be infected with T vaginalis. Prevalence estimates vary between populations studied, but range from 5–74% in women and 5–29% in men, with the highest rates reported in either sex from STI clinics and in other high risk populations.2–5 In men, the prevalence is less well described because many men are asymptomatic and may not seek evaluation. Furthermore, the diagnosis is less commonly sought in men.6 In either sex, concomitant infection with other sexually transmitted organisms occurs commonly.7,8 Because of the public health importance of this common STI, we review here the clinical aspects of trichomoniasis.


Using combinations of the keyword “trichomoniasis” with “epidemiology,” “diagnosis,” “manifestations,” and “therapy,” a Medline search was performed. The search was limited to English language articles published in the previous 10 years. Editorials, letters to the editor, case reports, and case series were excluded. The resulting titles were searched for and limited to “trichomoniasis” or “Trichomonas vaginalis.” Resulting abstracts were reviewed and articles were excluded if the focus was not clinically relevant. Articles representing a developing body of literature were limited to the most recent publication date.


A total of 396 articles were retrieved through the Medline search. Subsequent review by the authors resulted in 195 articles with “trichomoniasis” or “T vaginalis” in the title. Abstracts (n = 94) were reviewed; 52 of these were selected and reviewed here.


Among US women, race has been identified in several studies as predictive of infection with T vaginalis.9 This association is probably multifactorial, including access to care, personal health practices, and socioeconomic factors.1 Older age, histories of previous STI, prostitution, pregnancy, and drug use have also been associated with trichomoniasis.10,11 The association with older age is in contrast with the association between age and other STIs, especially chlamydia. Reproductive hormone levels may be partly responsible for higher prevalence of trichomoniasis in older women.12 Among African women, vaginitis including trichomonal vaginitis was significantly associated with sexual debut at less than 20 years, a partner who travelled frequently, history of STI, and presence of abnormal discharge.13 Concomitant STI diagnosis is common (15–28% chlamydial co-infection and 10% gonorrhoeal co-infection rates) in women with trichomoniasis, indicating the importance of screening for other STIs.14,15

Among US men attending STI clinics, the combination of age ⩾30 years, discharge, and diagnosis of non-gonococcal urethritis (NGU) was predictive of infection with T vaginalis, although this finding was not supported in a separate study.16,17 Sexual exposure to a woman with trichomoniasis and history of trichomoniasis were also associated with trichomoniasis in multivariate analyses.16 As in women, concomitant infection with other urethral pathogens is common in trichomoniasis. Mixed infections with T vaginalis and Neisseria gonorrhoeae and/or Chlamydia trachomatis occurred in seven of 980 rural Tanzanian men, while 61.5% of 91 men with trichomoniasis in west Africa also had a gonococcal infection.8,18,19

Finally, trichomoniasis facilitates spread of the HIV epidemic. One theoretical calculation concludes that if infection with T vaginalis increased the risk of HIV transmission by 90% in a population with 25% prevalence of trichomoniasis, approximately 20% of cases of HIV would be attributable to trichomoniasis.1 This relation could explain some of the differences in rates of HIV infection in different regions of Africa where rates of trichomoniasis and HIV seem to be directly proportional.20

Clinical presentation


T vaginalis is site specific for the genitourinary tract and has been isolated from virtually all genitourinary structures. However, many women diagnosed with trichomoniasis are asymptomatic; in Zimbabwe 75% of women denied symptoms on direct questioning and 16% of these women had trichomoniasis.13 When symptoms arise, the most common presenting complaint among women diagnosed with T vaginalis is vaginal discharge, seen in more than 50% of cases, followed by pruritus or dysuria. One study of 200 Nigerian women demonstrated 74% with vaginal discharge were infected with T vaginalis.2 On speculum examination, the vaginal discharge may be of any colour or characteristic, and although a frothy green discharge has classically been associated with trichomoniasis, this was not observed in any of 149 Nigerian women with discharge caused by trichomoniasis.2 The discharge may be malodorous with pH >4.5.7 Trichomoniasis has been associated with bacterial vaginosis in other studies.21,22 Clue cells were found to be an independent risk factor for trichomoniasis among 249 South African women attending family planning clinics.23 However, these characteristics are neither sensitive nor specific. The clinician’s ability to predict vaginal infection with T vaginalis based solely on physical examination was shown to have a positive predictive value of 47% among commercial sex workers in Cameroon.24

Cervical pathology can be commonly seen with trichomoniasis. Colpitis macularis, or “strawberry cervix,” (fig 1) results from microscopic, punctate haemorrhages of the cervix. T vaginalis is significantly associated with the clinical finding of colpitis macularis. Cervical mucopurulence, erythema, and friability have been observed.7,23T vaginalis may be associated with cervical cancer; however, confounding factors, such as co-infection with HPV, have not been well controlled for in some of these studies.25 Among HIV infected women, T vaginalis infection was associated with PID.21

Figure 1

Colpitis macularis, or “strawberry cervix.” (Courtesy, CDC.)

Trichomonal infections are neither restricted to nor exclusive for the vagina, and urinary tract infection may be common. Dysuria was observed in 29% of South African women diagnosed with T vaginalis infection.23 Thus, consideration should be given to the presence of trichomoniasis for women with dysuria as a sole complaint.

Finally, there is some suggestion that women may harbour T vaginalis for 3–5 years.26 The reasons for this are unclear, but may include imperfect screening tools, genitourinary anatomy, or type of infecting strain.


Less is known about T vaginalis infection in men. Current estimates of asymptomatic infection in men vary. In one US study, 23 of 50 patients with trichomoniasis were asymptomatic.27 Among 23 east African men with trichomoniasis, 19 were asymptomatic.28 Finally, among 100 men presenting for non-genitourinary complaints in the Central African Republic, seven were diagnosed with trichomoniasis.8 When symptoms of trichomoniasis become manifest, discharge may be less profuse and purulent than that seen with gonococcal infection.27 Men may also complain of dysuria. Of 103 Tanzanian men with trichomoniasis, dysuria occurred in 42%; only five men complained of urethral discharge.19 Finally, trichomoniasis was diagnosed in 66 of 410 men from the Central African Republic with urethral discharge.8 In men, urethral discharge and dysuria are the most common symptoms; however, some men diagnosed with trichomoniasis are asymptomatic.29 Many men clear T vaginalis from the genitourinary tract within 2 weeks.30 Involvement of other male genitourinary structures has been described, but will not be reviewed here.



Suitable clinical specimens include urine, vaginal fluid, semen, and endocervical smear. Because of discordantly infected anatomical sites among men, testing from multiple sites is advisable.31 Vaginal specimens for culture may be either self collected with a swab, or collected by the clinician with a swab or plastic loop.32 Self collected vaginal specimens, which may have greater acceptability with patients, perform similarly to clinician acquired specimens for the diagnosis of trichomoniasis.33

Direct visualisation

These methods depend on identification of the organism on microscopic examination of clinical specimens. Wet mount preparation and culture rely on visual detection of sufficient numbers of viable organisms with characteristic motility. Wet mount preparation has been the most commonly used method for diagnosis of trichomoniasis in women. Vaginal secretions are obtained from the lateral walls and fornices using a swab or plastic loop; in men, any urethral discharge, prostate secretions, or urethral scrapings may be used. The secretions are then suspended in 0.85% normal saline and examined under the microscope. Because the organism will quickly lose its characteristic tumbling motility, the specimen should be examined as quickly as possible after it has been obtained.34 Although this method has imperfect sensitivity, it is both inexpensive and rapidly performed.35

Culture has long been considered the gold standard for diagnosing T vaginalis infection. Several different media are available for culture, including self contained systems. Of these media, Diamond’s modified has been shown to be superior although the self contained system has been shown to have equivalent performance.36 Culture is more sensitive than wet mount preparation, because of the lower minimum concentration of organisms required for a positive result.36 The drawbacks to culture include greater cost and prolonged time to diagnosis (3–7 days). Screening with culture, although far more sensitive than wet mount preparation, may not be feasible; however, delayed inoculation after a negative wet mount preparation could increase detection in high prevalence populations without substantially increasing cost.37

As a screening tool for trichomoniasis, Papanicolaou (Pap) smear performs with low sensitivity (61%).38 In one study, endocervical smears were less sensitive than ectocervical smears, leading the authors to suggest that the ectocervical smear may have greater utility for detecting T vaginalis on Pap smear.39 Depending on the prevalence of T vaginalis in a population, almost one third of women with Pap smears positive for trichomoniasis receive unnecessary therapy.40 In a meta-analysis of the performance of Pap smear compared to wet mount preparation for the diagnosis of vaginal trichomoniasis, a three tiered approach for the interpretation of a positive Pap smear was proposed.41 In high prevalence populations (>20%), a positive Pap smear had a positive predictive value (PPV) of 83%, whereas in lower prevalence populations the PPV decreased. Confirmatory culture for T vaginalis has been recommended for suspicious Pap smears in low prevalence populations.41

Other staining and molecular probe methods have been employed with direct clinical specimens.42,43 While rapidly performed and having sensitivity similar to that of wet mount preparation, they require access to specialised equipment, skilled laboratory personnel, and sufficient financial resources.

Nucleic acid amplification

Because of the insensitivity of wet mount preparation and the problems with culture methods, nucleic acid amplification methods, such as polymerase chain reaction (PCR), have become increasingly attractive for diagnosing infection with T vaginalis. PCR has the advantage of requiring only DNA, from either viable or non-viable organisms, and in concentrations as low as one organism per PCR reaction.44

PCR has been performed on several different types of clinical specimens, most notably vaginal fluid and urine. Self administered vaginal swabs have been used for PCR assays, and performed at least as well as wet mount preparation, although specificity is less than that of wet mount or culture.45 Urine based PCR detection of T vaginalis has performed with sensitivity and specificity of 64–90.8% and 93.4–100% in US women and 92.7% and 88.6% in African men, respectively.46,47 This technology is not yet commercially available.

Syndromic management

Screening for trichomoniasis may be problematic in resource challenged settings. In such settings, the World Health Organization (WHO) has promoted syndromic approaches for managing STIs. However, using mathematical modelling, Bowden et al demonstrated that syndromic management had minimal effect on endemic prevalence, and that screening was the most efficient method of control.26 In general, syndromic management has been shown to work well for genital ulcer disease for either sex, and urethral discharge for men. The urethral discharge algorithm does not consider trichomoniasis until after the patient has been treated for gonorrhoea and chlamydia. Based on the prevalence of trichomoniasis as a cause of urethritis, some experts have suggested the addition of metronidazole or another nitroimidazole to the initial therapy in the syndromic management algorithm for urethral discharge.19 Syndromic management of vaginal discharge performs poorly for women because cervical discharge is a confounding factor. In a study of 249 South African women attending antenatal clinics, only one in three women treated for trichomoniasis based on a risk score would actually have been correctly diagnosed with trichomoniasis based on wet mount microscopy; overtreatment using syndromic management compared to microbiological diagnosis of trichomoniasis was observed in another study.23,48


Definitive therapy for trichomoniasis became possible with the introduction of nitroimidazoles (metronidazole, tinidazole, ornidazole, carnidazole, and nimorazole). In a meta-analysis of nitroimidazoles where the majority of studies used tinidazole or metronidazole for treatment of trichomoniasis, in short or prolonged courses of oral therapy, parasitological cure resulted in 90% of cases.49 Topical metronidazole is not effective for therapy for trichomoniasis.50 The Centers for Disease Control and Prevention (CDC) has estimated that 5% of clinical isolates of T vaginalis exhibited some degree of metronidazole resistance.51 Metronidazole resistance may predict tinidazole resistance; however, it is unclear if this situation is true for the entire class of nitroimidazoles.52 The CDC has proposed an escalated therapeutic regimen for management of infection with resistant strains (fig 2). Furazolidone, a nitrofuran, was shown to be effective in vitro against both laboratory and clinical specimens with high level metronidazole resistance, and topical paromomycin was effective in seven of 12 patients infected with resistant strains; however, paromomycin had adverse dermatological effects.52,53 Alternatives for treatment of highly resistant trichomoniasis exist but have not been subjected to the rigours of clinical trials.

Figure 2

Guidelines for treating metronidazole resistant trichomoniasis.

Side effects of nitroimidazoles include a bitter metallic taste, nausea, and vomiting, a disulfiram-like reaction with ingestion of alcohol and some central nervous system effects. Despite the low doses required for treatment of trichomoniasis, side effects occur in more than half of patients, especially with the metronidazole 2 g dose.49

Other management issues

Treatment relapse usually represents re-infection by an untreated partner in the majority of cases, although newly recognised chronic or drug-resistant infections are possible.9 Re-infection is common, occurring in 36% of subjects in one study.11 Recommendations for concomitant treatment of the male partner have been made since the 1960s and are now the standard of care.54

Risk factors for re-infection with T vaginalis in HIV infected women have been evaluated. In a study of 411 HIV positive women, 36% were re-infected at least once with T vaginalis over a mean follow up of 3.5 years. Predictors of re-infection or recurrent infection in these women were age ⩾22 years and history of previous STI in multivariate analysis.11

Some pregnant women with trichomoniasis may present with premature rupture of membranes, preterm labour.55 However, a randomised placebo controlled trial of 615 asymptomatic pregnant women with trichomoniasis did not demonstrate a difference in rates of preterm birth between the treatment and placebo groups.56 Although the reasons for this finding are unclear, the authors speculated that inflammatory responses to dying organisms or release of a pathogenic virus might be responsible. Because of the possible association with adverse birth outcomes, some authorities recommend treating trichomoniasis in pregnant women.54 The use of metronidazole in pregnancy has been controversial because of its potential mutagenic and carcinogenic potential. Increased risks of birth defects or teratogenicity have not documented.57 For clinicians concerned about the possibility of effects on the fetus, intravaginal therapy with metronidazole or clotrimazole could be considered as alternatives; however, studies of these therapies have demonstrated low parasitological cure rates.50,58


T vaginalis infection is highly prevalent in sexually active populations. Trichomoniasis is clearly associated with significant public health problems, including HIV transmission. Disease presentation is variable and diagnosis can be difficult with the most commonly used methods. Certain sociodemographic and behavioural risk factors may assist in predicting infection. Treatment of trichomoniasis with one dose of metronidazole is highly effective, and should be delivered in conjunction with concomitant treatment of sexual partners.

A majority of women and a significant proportion of men with trichomoniasis are without any symptoms; these patients would thus escape detection and treatment under syndromic management recommendations. Control programmes must work towards increased screening in order to have any effect on the burden of STIs.


HS and ACS, identification of articles for review; HS and ACS, analysis and interpretation; HS, drafting of the article; ACS, MMH, MSC, critical revision for content; ACS, MMH, MSC, final approval; HS, collection and assembly of articles.

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