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Epidemiology poster session 1: STI trends: Vaginosis
P1-S1.29 Use of molecular sequencing to compare the vaginal microbiota of healthy women and women with bacterial vaginosis in India
  1. S Kotian1,
  2. A Arun1,
  3. K Krupp1,
  4. V Srinivas1,
  5. P Madhivanan1,
  6. L Riley2,
  7. E Raphael3,
  8. A Reingold4,
  9. J Klausner5
  1. 1Public Health Research Institute, Mysore, India
  2. 2School of Public Health, University of California, Berkeley, USA
  3. 3University of California, Berkeley, Berkeley, USA
  4. 4School of Public Health, University of California USA
  5. 5University of California, San Francisco USA

Abstract

Background Lactobacillus species is an integral part of vaginal microbiota that maintains a healthy environment and plays an important role in preventing STI and HIV. We examined 20 women to investigate the difference in the diversity of Lactobacillus species present when the women are healthy or have Bacterial Vaginosis (BV).

Method Between February and November 2010, samples from a total of 20 women attending Prerana Women's Health Clinic were collected. Out of 20, 10 women were considered healthy and 10 women were diagnosed with BV based on Amsel's Criteria. In addition, Gram stained smears of vaginal fluid were Nugent scored as negative, intermediate, or positive for BV. Based on the Nugent score criteria, nine were Positive, nine were negative and two showed “Intermediate” Nugent Score. Vaginal swabs were taken from the women with informed consent after ethical approval and grown in MRS broth. Gram positive Lactobacilli generating about 600–800 bp amplicon by16SrDNA PCR were further characterised by sequencing.

Result Lactobacillus crispatus (40%) and Lactobacillus jensenii (40%) were the most common Lactobacillus species found in the vaginas of healthy women, the same Lactobacillus species found in healthy women in other countries. L crispatus was cultured from 40% of healthy women and none of women with BV. L jensenii, L gasseri, and L acidophilus were cultured from 40%, 10% and 10% of healthy women respectively; and none of the women with BV. Lactobacillus iners was not detected among healthy women or women with BV in our sample. Other organisms found among women were Staphylococcus epidermidis (60% among women with BV and 30% among healthy women), Streptococcus anginosus (40% among women with BV and 20% among healthy women). Some Corynebacterium spp were common among both women with BV and healthy women. Among the two women with “Intermediate” nugent score, one did not show growth of any Lactobacillus and in the other case there was growth of Lactobacillus salivarius.

Conclusion Our findings showed Lactobacilli species present in healthy vagina of women in India do not differ from those reported from other countries. This information is useful for the development of microbicides for HIV prevention as well as better understanding of the reproductive health of women in India.

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