Objective To evaluate a real-time PCR assay for molecular screening of five most common sexually transmitted diseases Trichomonas vaginalis, Mycoplasma genitalium, Neisseria gonorrhoeae, Chlamydia trachomatis and Herpes simplex virus 2 in Abidjan, Ivory Coast.
Methods 25 self-collected vaginal swabs were obtained from adult persons with symptoms of vaginal discharge and vulval irritation. The samples were diluted in sterile PBS and the DNA extraction was processed using Nuclisens extraction Kit. 5 μl of genome extract was applied in real-time PCR for each pathogen. The targeted region of real time was the 18 S ribosomal DNA genes for T vaginalis and the 16S rRNA for M genitalium.
Results 10 of 25 (40%) samples were positive for sexually transmitted dieases. Over 30 % of the samples shown inhibition and were tested positive by serial dilution. Chlamydia trachomatis is the most detected infection in five samples, and one sample was positive respectively by T vaginalis and by N gonorrhoeae. We detect no co-infection in all samples.
Conclusions The real-time PCR is a suitable specific and sensitive method to investigate and to detect sexually transmitted diseases in developing countries.