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P2.191 DC-SIGN,DC-SIGNR and SDF-1 Polymorphism in High Risk Seronegative Sexually Transmitted Disease Patients from North Indian
  1. O Chaudhary1,
  2. M Bala2,
  3. J Singh3,
  4. A Hazarika4,
  5. K Luthra5
  1. 1All India Institute of Medical Sciences, New Delhi, India
  2. 2Regional STD Teaching, Training & Research Centre, Safdarjang Hospital, New Delhi, India
  3. 3Deptt. of Biochemistry, Kurukshetra University, Kurukshetra, India
  4. 4Blood Transfusion Services, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences, New Delhi, India
  5. 5Deptt. of Biochemistry, All India Institute of Medical Sciences, New Delhi, India

Abstract

Background Dendritic cells are the first to encounter HIV-1 at mucosal sites and virus binding occurs via receptors known as DC-SIGN/R. Variations in the number of repeats in the neck region of DC-SIGN/R are reported to possibly influence host susceptibility to HIV-1 infection A single nucleotide polymorphism (SNP) in SDF-1, the natural ligand for the HIV-1 co-receptor CXCR4, is implicated to have protective effects against HIV-1 infection.

Methods The repeat region polymorphisms in DC-SIGN/R by PCR and SNP of SDF1–3’A by PCR-restriction fragment length polymorphism (RFLP) in 230 healthy HIV seronegative individuals, 200 high risk sexually transmitted disease (STD) patients seronegative for HIV and 230 HIV-1 seropositive patients from northern India. The study was approved by the institutional ethics committee.

Results The frequency of homozygous DC-SIGNR 7/7 genotype and allele 7 was significantly higher in patients infected with HIV-1 (P < 0.0001) whereas frequency of heterozygous DC-SIGNR 7/5 genotype and allele 5 was significantly higher in high risk STD patients seronegative (P = 0.003). The heterozygous DC-SIGNR genotypes 7/5 and allele 5 was associated significantly with high CD4+ T-cell count and low viral load compared to homozygous DC-SIGNR 7/7 genotype and allele 7 in patients infected with HIV-1. DC-SIGN genotype 7/7 was most frequent in all three groups. A significantly higher frequency of SDF1–3A/SDF1–3A was observed in high risk STD patients as compared to HIV seropositive (p = 0.005) and healthy HIV-1 seronegative tested individuals (p = 0.001).

Conclusion The significant higher frequency of heterozygous DC-SIGNR 7/5 and SDF1–3A genotypes in high risk STD patients and with high CD4+T-cell count and low viral load in HIV-1 seropositive patients suggesting the protective role of this genotype in HIV-1 infection.

  • North India
  • PCR
  • Polymorphism

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