Article Text

PDF

P3.022 Chlamydia Trachomatis (Ct) Infections: False Negative PCR-Testing in Cryptic Plasmid Deleted Ct Can Be Easily Detected Using a MOMP-Analysing PCR
  1. M T Marker
  1. Department of Dermatology Rudolfstiftung, Vienna, Austria

Abstract

Background Ctis globally the most common cause of sexually transmitted infections. A new variant of ct with a deletion in the cryptic plasmid has been found in Sweden, following an unexpected 25% increase in genital infections in 2006. This variant escapes routine diagnostic PCR-tests. Thus a new nuclear acid amplification test (NAAT), which uses the cryptic plasmid as well as the MOMP-gene as target area was developed. The MOMP-gene encodes a protein (OMP-1) which represents 60% of the proteins embedded in the peptidoglycans of the bacterial cell wall. The aim of this study was to define the number of cryptic plasmid-/MOMP+ patients.

Methods Between 2009 to 2012 we analysed probes of 11250 individuals (patients and controls) processing the ProbeTecET® test (BD, USA). Of these 407 showed a positive result and were treated according to current guidelines. 33 patients tested negative, however, reported a persistence of discomfort such as burning sensations in the urethra, urethral discharge and occasionally conjunctivitis. These patients were additionally tested with the GenoQuick® CT (HAIN Lifescience, Germany), which specifically and simultaneously detects both, the MOMP-gene and the cryptic plasmide. Material was taken from urethral, cervical, rectal, pharyngeal, conjunctival smears and from the Douglas-space.

Results All 33 patients tested positive when processing the GenoQuick® CT. Thus 7.5% of infected patients were only identified processing an additional detection set.

Conclusion In our centre 7.5% of ct infected patients were tested “false negative” when only the cryptic plasmide was analysed. These 33 patients were identified processing a more sensitive test system and subsequently were treated.

  • Chlamydia trachomatis
  • cryptic plasmide
  • MOMP-gene

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.