Background One approach to eliminating sexually transmitted Infections (STIs) in a community is to screen high-risk persons, followed by the treatment and education of people who test positive. We examined a Triplex PCR assay to detect urine samples in pregnant women. major goal of this study was to determine the prevalence of Chlamydia trachomatis (Ct.), Neisseria gonorrhoeae (Ng.) and Ureaplasma Urealyticum (Uu.) and to apply a Triplex PCR techniques to detect 3 pathogens in one specimens.

Methods A cross-sectional study was conducted among 399 pregnant adolescents women. All participants received prenatal care and delivered at urban hospital in Sabzevar- Iran. A socio- demographic questionnaire was completed. A Triplex PCR with universal primers was developed for screening of subjected women in this study. Descriptive and univariate analyses were performed to describe disease prevalence.

Results Of pregnant adolescents, 15.28% were diagnosed with either Ct., Ng. or Uu.infection. The prevalence of Ct. was 12.28%, and that of Ng 1.25% and finally 19.54% of pregnant had Uu. infection.

In univariate analysis, Ct. was associated with having had any level of education (P < 0.05), abortion (P < 0.05), and Uu. was associated with PTD (P < 0.05). Multivariate analysis did not show any significant association.

Conclusions Compared with available data, a decline of STIs prevalence was observed in our setting. This might be the result of community-based education programmes focusing on changes to sexual behaviour. However, STIs rates are still high, and the problem needs more concrete and sustained efforts for its control. Screening for Ct., Ng. and Uu.is recommended during pregnancy. Based on our finding in this study, the overall high incidence of Ct.and Uu. support screening recommendations for pregnant women in Sabzevar- Iran.