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P2.016 Comparison of Three Real-Time PCR Tests For the Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Young Pregnant Women
  1. O Peuchant1,2,3,
  2. S de Diego1,
  3. C Le Roy1,2,
  4. S Frantz-Blancpain3,
  5. C Hocké1,3,
  6. C Bébéar1,2,3,
  7. B de Barbeyrac1,2,3
  1. 1University of Bordeaux, Bordeaux, France
  2. 2Institut National de la Recherche Agronomique, Bordeaux, France
  3. 3Centre hospitalier universitaire, Bordeaux, France

Abstract

At the Bordeaux University Hospital, among pregnant women aged less than 25 years-old the prevalence of C. trachomatis (CT) and N. gonorrhoeae (NG) is 12.5% and 2.4%, respectively for those requesting abortion by using the Abbott m2000 CT/NG test, and 7.9% and 0%, respectively, for those consulting for monitoring pregnancy by using the Roche Cobas 4800 CT/NG test.

The objective of this study was to evaluate and compare the performances of both of these tests along with a third one, the Cepheid GeneXpert CT/NG, for the detection of CT and NG in vaginal swabs collected from pregnant women consulting for abortion or for monitoring pregnancy. A patient was considered infected if at least two tests were positive.

Among 304 pregnant women included, from September 2012 to January 2013, 34 were infected, leading a prevalence of 11.7% (26/222) for patients requesting abortion and 9.7% (8/82) for patients consulting for monitoring pregnancy. There were two false CT-positive results with the Abbott m2000, one false negative result reported by Roche and the Cepheid GeneXpert. The sensitivity and specificity were 97% and 100% for the Roche and the Cepheid GeneXpert tests, and 100% and 99.3% for the Abbott assay. The positive predictive value ranged from 94.4% to 100% according to the test.

For NG, 297 specimens were negative and 7 were positive using the three tests. All results were concordant, leading to a sensitivity and specificity of 100% for all the assays. The prevalence of NG among pregnant women requesting abortion was 2.7% (6/222) and 1.2% (1/82) for those consulting for monitoring pregnancy.

In the populations studied, all three assays have similar performances for CT/NG detection.

  • Chlamydia trachomatis
  • Neisseria gonorrhoeae
  • young pregnant women

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