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O15.6 Impact of the lng-ius on cervical persistence of chlamydia trachomatis and vaginal microbiota in a baboon model
  1. ER Liechty1,
  2. IL Bergin1,
  3. CM Bassis2,
  4. D Chai3,
  5. W LeBar4,
  6. VB Young2,
  7. JD Bell5
  1. 1University of Michigan, Unit for Laboratory Animal Medicine
  2. 2University of Michigan, Department of Internal Medicine, Division of Infectious Diseases
  3. 3Institute for Primate Research
  4. 5University of Michigan, Department of Obstetrics and Gynecology
  5. 4University of Michigan, Department of Pathology

Abstract

Introduction Alterations in vaginal microbiota associated with intrauterine contraception may impact host susceptibility to sexually transmitted infection. We evaluated the effect of the levonorgestrel intrauterine system (LNG-IUS) on cervical persistence of Chlamydia trachomatis (CT) in a baboon model and whether CT persistence was correlated with vaginal microbial community structure.

Methods 20 wild caught female olive baboons (Papio abubis) were randomly assigned to receive either LNG-IUS and CT inoculation (n = 8), LNG-IUS and sham inoculum (n = 2), CT inoculation alone (n = 8), or sham inoculation (n = 2). Animals were acclimated to the LNG-IUS for 24 weeks after which animals were cervically inoculated once weekly for 5 weeks. Vaginal swabs were collected weekly for microbiome analysis by 16S rRNA-encoding gene sequence analysis. Presence of CT in the cervical epithelium was confirmed with weekly nucleic acid amplification testing (NAAT) and culture.

Results Use of the LNG-IUS was significantly associated with positive CT culture (p = 0.04) but not NAAT (p = 0.07). Median time to cervical clearance of CT as detected by NAAT was 12.5 days (range 5–16) for LNG-IUS animals in comparison to 7 days (range 3–10) for non-implanted animals (p = 0.14). Similarly, median time to cervical clearance of CT by culture was 12 days (range 5–15) for LNG-IUS animals and 5 days (range 1–10) for non-implanted animals (p = 0.05). We did not detect significant within group differences between vaginal microbial community structure at baseline and following LNG-IUS insertion, CT inoculation, or LNG-IUS and CT in combination.

Conclusions Use of the LNG-IUS was associated with a trend towards cervical persistence of CT in a baboon model. However, this persistence is not explained by alterations in vaginal microbial communities.

Disclosure of interest statement The authors have no disclosures to report.

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