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O021 A Question of Stability
  1. Hemanti Patel1,
  2. Gabriel Schembri2,
  3. Binta Sultan3,
  4. Catherine Ison4
  1. 1Public Health England, London, UK
  2. 2Manchester Centre for Sexual Health, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK
  3. 3Mortimer Market Centre, Central and North West London NHS Foundation Trust, London, UK
  4. 4Bacterial Special Interest Group, London, UK

Abstract

Background/introduction Urines to be tested by the APTIMA Combo 2 (AC2) are added to a collection tube containing preservatives to ensure stability of the nucleic acid for testing within 24 hours of collection. Home collected urines are often collected in containers without preservative to avoid the patient manipulating the sample.

Aim(s)/objectives An investigation was undertaken to determine the stability of gonococcal and chlamydial nucleic acids within neat urine stored in different conditions over a period of 25 days to provide evidence of the stability of the nucleic acid prior to testing.

Methods To mimic collection in a home setting and differing nucleic acid loads within clinical specimens, uninfected urine was inoculated with different concentrations of chlamydial (from cell culture) and gonococcal (from bacterial culture) nucleic acid. Aliquots of the urine were removed on eight occasions over 25 days, added to collection tubes and tested either on the Hologic Panther system to determine presence of RNA or, following DNA extraction, using in-house PCRs to determine DNA load.

Results Chlamydial RNA and DNA remained stable for over three weeks when either refrigerated or stored at room temperature. Gonococcal RNA was detectable up to three weeks if refrigerated and two weeks if stored at room temperature. GC DNA was detectable for 18 days if refrigerated and for 11 days if stored at room temperature.

Discussion/conclusion Chlamydial and gonococcal nucleic acids are stable in urine before addition to preservatives for longer than recommended by the manufacturer, enabling more flexibility for home collected samples.

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