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P3.35 Frequency of human papillomavirus and genotypes in population attending a women`s clinic in monterrey, mexico
  1. N Casillas-Vega,
  2. A Flores-Aréchiga,
  3. S Lozano-Quintanilla,
  4. F Pérez-Chávez,
  5. Llaca-Díaz J1
  1. Universidad Autónoma de Nuevo León, Monterrey, Mexico


Introduction Human papillomavirus (HPV) is the most common viral infection of the reproductive tract. The aim is to determine the frequency of HPV and its genotypes in population that came to a Women’s Clinic.

Methods HPV detection was performed in endocervial samples from 339 patients that came to woman clinical in the Hospital ”Dr. José Eleuterio González“. DNA extraction was performed; After that, the β globin gene was detected to validate the presence of epithelial cells in the sample; HPV was detected by the PCR technique and nucleic acid hybridization; 17 high-risk genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73 and 82) and 17 low-genotypes (6, 11, 26, 40, 42, 54, 55, 61, 62, 64, 67, 69, 70, 71, 81, 83 and 84).

Results The mean age of the population was 50 (range, 18–89) years. Of the samples that were positive for the β globin gene (n=316); 7% (n=22) was positive for HPV. 18 HPV genotypes were detected; of which 65% (n=10) were at high risk. The most frequent genotypes were 16 (n=4), 59 (n=4), 51 (n=3) and 42 (n=3). In 77% (n=17) of the patients, high-risk genotypes were detected. In 59% (n=13) of the population a 1 genotype was detected, in 23% (n=5) 2 genotypes and in 18% (n=4) 3 genotypes.

Conclusions Of the 22 patients infected with HPV, 17 had been infected with at least one high-risk genotype. The most frequently detected genotypes were 16 and 59.

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