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P3.164 Antibiotic resistance and molecular typing of neisseria gonorrhoeae isolated from the three overseas sites through the global emerging infections surveillance and response system (GEIS)
  1. Nazia Rahman1,
  2. Nicole Kluz2,
  3. Naiki Puplampu-Attram3,
  4. Andrew Letizia3,
  5. Silvia Montano4,
  6. James Regeimbal4,
  7. Andrea Mccoy4,
  8. Tamar Akhvlediani5,
  9. Michael Washington5,
  10. Olusegun Soge6,
  11. Grace Macalino1,
  12. Ann Jerse1
  1. 1Uniformed Services University, Bethesda, Md, USA
  2. 2University of Texas, Austin, Tx, USA
  3. 3U.S. Naval Medical Research Unit Number 3, Accra, Ghana
  4. 4U.S. Naval Medical Research Unit Number 6, Lima – Peru
  5. 5U.S. Army Medical Research Unit, Georgia, Tbilisi, Georgia
  6. 6University of Washington, Seattle, USA

Abstract

Introduction Increasing antibiotic resistance in Neisseria gonorrhoeae (GC) threatens treatment and control measures for gonorrhoea and can affect military readiness. The Global Emerging Infectious Surveillance and Response System of the US Armed Forces Health Surveillance Branch supports a repository for GC isolated at US military treatment facilities in the continental US (CONUS) and at several overseas (OCONUS) labs. Here we report the antibiotic susceptibility and N. gonorrhoeae multi-antigen sequence types (NG-MAST) of isolates collected from three OCONUS sites: Republic of Georgia, Peru and Ghana in 2012–2016.

Methods GC was identified using standard biochemical and serological methods. Susceptibility to ceftriaxone, cefixime (Cfx), azithromycin (Az), gentamicin, penicillin (Pen), tetracycline (Tet), ciprofloxacin (Cip), and spectinomycin was determined by Etest. β-lactamase (ßL) activity was determined by nitrocefin hydrolysis. NG-MAST was performed using standard methods.

Results Fifty-seven confirmed GC isolates were obtained from the three OCONUS sites. Cip resistance occurred in 74% (Ghana) and 89% (Peru) of isolates, with 63.2% (Ghana) and 28.6% (Peru) of isolates CipR, PenR and TetR. Two isolates with reduced susceptibility to Cfx were identified among isolates from Ghana and Georgia and 18 isolates with reduced susceptibility to Az were identified across the 3 sites. Over 65% of isolates from Ghana and Peru produced ßL. CipR strains primarily encoded S91,D95A or S91F,D95G substitutions in GyrA, combined with S87R (Peru) or S87N (Ghana) substitutions in ParC. Interestingly, a high proportion of isolates from Ghana (36.8%) and Peru (85.7%) were of unique NG-MAST types.

Conclusion Multidrug resistant GC and ßL production are common in these OCONUS sites. Several previously undescribed NG-MAST sequence types were identified in Peru and Ghana, suggesting the GC strains circulating in these countries are different from those in North America, Australia, and Europe. Further surveillance is needed to inform treatment recommendations in OCONUS sites.

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