Objectives: Establishing a connection between the emerging urogenital tract pathogen Mycoplasma genitalium and upper genital tract infection in women is of major importance. The aim of this study was to evaluate the association between M. genitalium antibodies and Pelvic Inflammatory Disease (PID) and Ectopic Pregnancy (EP) using a Lipid Associated Membrane Protein - Enzyme Immuno Assay (LAMP-EIA) method.
Methods: The LAMP-EIA was used to analyse sera obtained from patients with clinical PID and EP collected in Sweden, from February 1984 to April 1986. A control-group consisting of healthy pregnant women (Cp) collected during approximately the same time period was used. Evidence of chlamydial infection was detected using a commercial anti- Chlamydia trachomatis EIA assay.
Results: The LAMP-EIA was specific as determined from lack of cross-reactivity to other Mycoplasma species. Of 193 PID patients 33 (17%) were M. genitalium LAMP-EIA positive as compared to 18% (15/82) of the EP patients, and 15% (36/246) of the Cp women. No significant association could be demonstrated between M. genitalium antibodies and PID or EP in crude or adjusted logistic regression. Antibodies against C. trachomatis were demonstrated in 54% of the PID and 57% of the EP patients but also in 37% of the Cp women showing a statistically significant association.
Conclusion: No statistically significant association between PID or EP and M. genitalium antibodies in the LAMP-EIA could be found, although a slight tendency toward association was found when focusing on younger individuals.
- Ectopic pregnancy
- Mycoplasma genitalium
- Pelvic inflammatory disease