Article Text

other Versions

PDF
Genotype distribution of Candida albicans strains associated with different conditions of vulvovaginal candidiasis, as revealed by microsatellite typing
  1. S. R. Fan (fanshangrong{at}21cn.com)
  1. Peking University First Hospital, Beijing, 100034, China
    1. Q. P. Liao (qingping_liao{at}hotmail.com)
    1. Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, 100034, China
      1. J. Li
      1. Institute of Microbiology, Chinese Academy of Sciences, China
        1. X. P. Liu
        1. Peking University Shenzhen Hospital, Shenzhen, China
          1. Z. H. Liu (xiaopingliu{at}yahoo.com.cn)
          1. Department of Obstetrics and Gynecology, Peking University First Hospital, China
            1. F. Y. Bai (baify{at}im.ac.cn)
            1. Institute of Microbiology, Chinese Academy of Sciences, China

              Abstract

              Objective: To compare genotypes of Candida albicans strains causing different conditions of vulvovaginal candidiasis (VVC) in Chinese women.

              Methods: C. albicans strains were isolated from the vagina of patients with different conditions of VVC. The genotypes of the strains were investigated based on single-strand conformation polymorphisms (SSCP) of PCR amplified microsatellite CAI.

              Results: A total of 93 independent C. albicans strains were isolated from the patients with mild-to-moderate (n = 37) or severe (n = 56) VVC. Thirty of the patients enrolled suffered recurrent VVC (RVVC). Twenty-six distinct genotypes tentatively designated as A to Z were identified from the 93 C. albicans strains compared based on their CAI SSCP patterns. The majority (72.0%) of the strains possessed genotypes A to D which were similar in the CAI SSCP profiles and were designated as the dominant genotypes. The overall frequencies of the four dominant genotypes were 87.5% (49/56) and 48.6% (18/37) (p < 0.001) in the C. albicans strains from the patients with severe and mild-to-moderate VVC, respectively. The strains with the dominant genotypes occupied 83.3% (25/30) and 66.7% (42/63) (p = 0.094) in the C. albicans strains from the patients with RVVC and sporadic VVC, respectively.

              Conclusion: The frequency of C. albicans strains with the dominant genotypes (A to D) from the patients with severe VVC was significantly higher than that from the patients with mild-to-moderate VVC, implying that the CAI genotype distribution of C. albicans strains correlates with severity of VVC.

              • Candida albicans
              • SSCP
              • genotype
              • microsatellite
              • vulvovaginal candidiasis

              Statistics from Altmetric.com

              Request permissions

              If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.