Detection of people with acute HIV infection (AHI), the pre-seroconversion "window phase" of HIV infection, affords an important opportunity for early HIV treatment and prevention because HIV viral load usually peaks in blood and genital secretions during this time. Since people with AHI are unaware of their HIV status and will be HIV ELISA negative, they may substantially contribute to the spread of HIV. The use of a pooling algorithm with nuclear amplification assays to detect HIV RNA has been developed as a strategy for detection of AHI cases both in the US and developing countries.Sexually transmitted disease (STD) clinic patients would be at particularly increased risk for AHI.
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