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Sex Transm Infect doi:10.1136/sti.2007.029587

Validation of COBAS Taqman CT for the detection of Chlamydia trachomatis in vulvo-vaginal swabs

  1. Sue J Skidmore (sue.skidmore{at}sath.nhs.uk)
  1. Shrewsbury and Telford Hospital NHS Trust, United Kingdom
    1. Moira Kaye (moira.kaye{at}sath.nhs.uk)
    1. Shrewsbury and Telford Hospital Nhs Trust, United Kingdom
      1. Sinnathamby Devendra (sinnathamby.devendra{at}telfordpct.nhs.uk)
      1. Shrewsbury and Telford Hospital NHS Trust, United Kingdom
        1. Deborah Bayliss (deborah.bayliss{at}telfordpct.nhs.uk)
        1. Shrewsbury and Telford Hospital NHS Trust, United Kingdom
          • Published Online First 27 February 2008

          Abstract

          Background: Vulvo-vaginal swabs (VVS) are not validated for use by the manufacturers of two widely used Nucleic Acid Amplification Tests (NAAT) for the detection of Chlamydia trachomatis (CT). However, there is evidence that this type of swab is suitable for diagnosis.

          Objective: To validate the Cobas Taqman CT assay (Roche) for the detection of CT in VVSs.

          Method: Women aged 18-24 years attending a Genitourinary Medicine (GUM) clinic were invited to take part in the study. Participants provided a self-taken VVS and the results obtained with these samples were compared to those obtained with an endocervical swab collected by a healthcare worker. A total of 267 women took part

          Results: 255/267 (96%, 95%CI 92-98%) sets of samples gave concordant results. 12/267 (4.5%) VVSs were invalid/inhibitory and so no result was available for these samples. This compared to 2/267 (0.7%) for endocervical swabs.

          Conclusion: VVSs are suitable samples for detecting Chlamydia trachomatis.

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