Elsevier

Gynecologic Oncology

Volume 73, Issue 3, June 1999, Pages 359-361
Gynecologic Oncology

Regular Article
An Association Between LSIL and the High Secretor Phenotype of IL-1β

https://doi.org/10.1006/gyno.1999.5366Get rights and content

Abstract

Objective. The aims of this study were to determine the frequency of a nucleotide transition from C to T, which leads to increased transcription of interleukin-1β (IL-1β) in patients with different grades of cervical lesions, and to determine whether a correlation exists between the genotypes and cervical lesions.

Methods. One hundred forty-seven DNA samples from patients with different grades of cervical lesions were compared with 100 healthy, age and sex-matched bone marrow donors. TaqI restriction digest of PCR products was used to analyze the IL-1β +5887 C → T mutation and the results were confirmed using induced heteroduplex analysis with an induced heteroduplex generator.

Results. The Pearson χ2 test (Yate's correction) was used for statistical analysis. Patients with LSIL (n = 80) demonstrated a high frequency of allele T, previously associated with high IL-1β secretor phenotype, compared to controls (P = 0.000012). A trend was also observed in patients with HSIL (n = 28, P = 0.039).

Conclusions. We report for the first time a highly significant association between high secretor IL-1β phenotypes (i.e., IL-1β +5887 CT or TT genotypes) and LSIL. A less significant association exists with HSIL. The intrinsic ability to produce variable amounts of IL-1β during different clinical stages involving cervical lesions may be of immunological importance in their pathogenesis.

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