RT Journal Article SR Electronic T1 LB1.66 Detection of zika virus and cytomegalovirus in cervical cytology samples of pregnant women from guayaquil, ecuador, using two real-time polymerase chain reaction (RT-PCR) molecular assays JF Sexually Transmitted Infections JO Sex Transm Infect FD BMJ Publishing Group Ltd SP A68 OP A68 DO 10.1136/sextrans-2017-053264.171 VO 93 IS Suppl 2 A1 Héctor Zambrano A1 Jesse Waggoner A1 Karina León A1 Benjamin Pinsky A1 Marissa Schettino A1 Ketty Vera A1 Lissette Rivera A1 José Landivar A1 Gil Mor YR 2017 UL http://sti.bmj.com/content/93/Suppl_2/A68.1.abstract AB Introduction Zika virus (ZIKV) infection during pregnancy has been linked to severe birth defects. Human Citomegalovirus (CMV) has also been related to important congenital problems when present during pregnancy. The epidemiologic situation of the ZIKV epidemic and the prevalence of the CMV in Ecuador is poorly understood. Given the well-documented effects of ZIKV and CMV in pregnancy, we tested for the presence of both ZIKV and CMV in cervical cytology samples of pregnant women. We report the identification of a population of pregnant women with a high incidence of ZIKV infection and CMV infection in the lower reproductive tract.Methods In late 2016, a case control study was performed to determine the incidence of ZIKV infection and CMV infection among low-income, pregnant women at risk for preterm delivery compared to matched controls. Cervical cytology specimens were tested for ZIKV by rRT-PCR using a lab developed, clinically validated assay (ZCD assay) and for CMV using a commercial RT-PCR assay (CMV DiaPro).Results Fifty-nine pregnant women were enrolled. The incidence of ZIKV was 45.7% (27/59) overall: 15/31 (48.3%) in cases and 12/28 (42.8%) in controls. The general incidence of CMV was 37.2% (22/59): 12/31 (38.7) in cases and 10/28 (35.7) in controls. Overall, outcomes for neonates born to ZIKV-positive and ZIKV-negative mothers were similar. There were no significant differences in the outcomes of neonates born to CMV positive and CMV-negative mothers. However, two neonates were born with microencephaly to case mothers who were ZIKV-positive. Conclusion We report a high incidence of ZIKV infection (45.7%) and CMV infection (37.2) in a distinctive population in Guayaquil, Ecuador. We identify ZIKV and CMV in cervical samples. These data raise concerns regarding the breadth of the ZIKV epidemic in Ecuador and the importance of CMV infection in pregnant women. Our findings add to the body of evidence of female-male sexual transmission of ZIKV. This data demonstrate the utility of cervical cytology specimens for ZIKV and CMV testing.