Elsevier

Steroids

Volume 59, Issue 3, March 1994, Pages 205-211
Steroids

Paper
Validations of time-resolved fluoroimmunoassays for urinary estrone 3-glucuronide and pregnanediol 3-glucuronide

https://doi.org/10.1016/0039-128X(94)90029-9Get rights and content

Abstract

Competitive time-resolved fluoroimmunoassays (FIAs) were developed for measuring 1,3,5(10)-estratrien-3-ol-17-one glucosiduronate (estrone 3-glucuronide, E13G) and 5β-Pregnane-3α,20α-diol 3-glucosiduronate (pregnanediol 3-glucuronide, Pd3G) in unextracted urine. The assay are specific, detect 0.98 ng E13G/mL and 0.035 μg Pd3G/mL, measure 102.8 ± 2.0% of E1eG and 93.6 ± 2.9% of Pd3G added, and exhibit between and within assay coefficients of variation, respectively, of 5.3% and 7.1% for E13G and 6.8% and 7.8% for Pd3G. The urine matrix does not interfere with the assay. Urinary steroid glucuronide profiles measured by these FIAs conform to those of urinary steroid glucuronides and serum estradiol and progesterone measured by other established immunoassays. These FIAs afford the advantages of non-radioisotopic procedures and urine sample collection (convenience, non-invasiveness, integration of pulsatile secretion) to evaluate menstrual function in epidemiological, medical, and athletic populations

References (31)

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This work originated at the National Institute for Occupational Safety and Health (NIOSH). Mention of a trade name, proprietary product, or specific equipment in this manuscript does not constitute an endorsement, guarantee, or warranty by the NIOSH.

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