Detection of Chlamydia trachomatis DNA in joints of reactive arthritis patients by polymerase chain reaction

doi:10.1016/0140-6736(92)90399-N

The Lancet

Volume 340, Issue 8811, 11 July 1992, Pages 81-82

https://doi.org/10.1016/0140-6736(92)90399-N Get rights and content

Abstract

In 1986, Chlamydia trachomatis elementary bodies were found by direct immunofluorescence (DIF) in synovial-fluid cell deposits and synovial-membrane biopsy samples from five of eight patients with sexually acquired reactive arthritis (SARA) but in none of eight controls with other types of arthritis. Cells from the original slides (stored at 4°C) have now been examined by a polymerase chain reaction (PCR) that amplifies DNA for the major outer membrane protein of C trachomatis. Chlamydial DNA was found in samples from four DIF-positive patients, one DIF-negative patient, and one DIF-negative control. Overall, there was 80% concordance for DIF and PCR results. This study supports our previous finding of chlamydiae in joints in reactive arthritis.

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Citation Excerpt :
Chlamydia trachomatis is strongly associated with reactive arthritis. Nucleic acid from this organism has been detected in synovial fluid by direct immunofluorescence or polymerase chain reaction.6 Gastrointestinal pathogens such as Campylobacter jejuni, Clostridium difficile, Escherichia coli, Salmonella (various species), Shigella (especially S flexneri), and Yersinia (especially Y enterocolitica and Y pseudotuberculosis).
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SHORT REPORTSDetection of Chlamydia trachomatis DNA in joints of reactive arthritis patients by polymerase chain reaction

Abstract

Lancet

Role of Chlamydia trachomatis and HLA-B27 in sexually acquired reactive arthritis

Br Med J

Evidence that Chlamydia trachomatis causes seronegative arthritis in women

Ann Rheum Dis

Detection of Chlamydia trachomatis by the polymerase chain reaction in swabs and urine from men with non-gonococcal urethritis

J Clin Pathol

Small numbers of Chlamydia trachomatis elementary bodies on slides detected by the polymerase chain reaction

J Clin Pathol

SHORT REPORTS
Detection of Chlamydia trachomatis DNA in joints of reactive arthritis patients by polymerase chain reaction