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Alexander M. Hirschi, Manuela Richter, Athanasios Makristathis, Petra M. Prûckl, Birgit Willinger, Kurt Schûtze, Manfred L. Rotter, Single and Multiple Strain Colonization in Patients with Helicobacter pylori Associated Gastritis: Detection by Macrorestriction DNA Analysis, The Journal of Infectious Diseases, Volume 170, Issue 2, August 1994, Pages 473–475, https://doi.org/10.1093/infdis/170.2.473
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Abstract
Helicobacter pylori isolates from 18 patients were examined by macrorestriction DNA analysis to determine whether >2 strains can coexist in the human stomach. Four biopsy samples from different sites in each patient's stomach were cultured, and 12-14 clones of H. pylori per patient were analyzed. DNA of 15 of the 18 isolates was digestible: 12 by NotI and 3by NruI. Within 14 of these digestible isolates, the patterns of all clones were identical. The isolate of 1 patient showed two digestion patterns, a finding that was confirmed by arbitrary primer polymerase chain reaction. The typeable isolates of all patients, except 2 who underwent gastroscopy on the same day, differed markedly from each other. These results indicate a noticeable discrepancy between the pronounced interpatient heterogeneity and the rare intrapatient variation of H. pylori.