Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis

Alexander Vasilevich Kouznetsov; Peter Weisenseel; Paul Trommler; Susanne Multhaup; Joerg Christoph Prinz

doi:10.1016/j.diagmicrobio.2004.10.003

Detection of the 47-kilodalton membrane immunogen gene of Treponema pallidum in various tissue sources of patients with syphilis

Diagn Microbiol Infect Dis. 2005 Feb;51(2):143-5. doi: 10.1016/j.diagmicrobio.2004.10.003.

Authors

Alexander Vasilevich Kouznetsov¹, Peter Weisenseel, Paul Trommler, Susanne Multhaup, Joerg Christoph Prinz

Affiliation

¹ Laboratory of Immune Pathogenesis and Serology, Department of Dermatology and Allergology, Ludwig-Maximilians-University of Munich, 80337 Munich, Germany.

PMID: 15698722
DOI: 10.1016/j.diagmicrobio.2004.10.003

Abstract

Polymerase chain reaction (PCR) was used to detect the 47-kDa immunogen gene of Treponema pallidum in peripheral blood mononuclear cells (PBMCs), skin lesions, and serum, but less consistently in purified granulocytes or ejaculates of patients with manifest and latent syphilis. Therefore, skin lesions and PBMCs may serve as the most reliable sources for a PCR-based diagnosis of syphilis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carrier Proteins / chemistry
Carrier Proteins / isolation & purification*
DNA, Bacterial / analysis*
Female
Humans
Lipoproteins / chemistry
Lipoproteins / isolation & purification*
Male
Molecular Sequence Data
Polymerase Chain Reaction*
Sensitivity and Specificity
Skin / microbiology
Syphilis / diagnosis*
Syphilis, Cutaneous / diagnosis
Syphilis, Latent / diagnosis
Treponema pallidum / genetics*
Treponema pallidum / isolation & purification*

Substances

47-kDa immunogen, Treponema pallidum
Carrier Proteins
DNA, Bacterial
Lipoproteins