Abstract
Herpes simplex virus type 1 (HSV-1) Us11 protein, a true late gene product packaged within the virion, is delivered into cells after infection, exhibits a nucleocytoplasmic localization at early times, and later accumulates in the nucleoli. This RNA-binding basic phosphoprotein, capable of oligomerization, is supposed to be involved in post-transcriptional regulation of gene expression after HSV-1 infection. Expression of human T-cell leukaemia/lymphoma virus type-I (HTLV-I) and of human immunodeficiency virus type 1 (HIV-1) is post-transcriptionally regulated by Rex and Rev, respectively. These proteins are required for the cytoplasmic expression of unspliced gag-pol and singly spliced env transcripts. Here we show that HSV-1 Us11 protein is able to bind Rex- and Rev-responsive elements and to transactivate envelope retroviral glycoprotein expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cytoplasm / metabolism
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Gene Expression Regulation, Viral*
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Gene Products, rev / genetics
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Gene Products, rev / physiology
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Gene Products, rex / genetics
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Gene Products, rex / physiology
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Gene Products, tax / genetics
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Giant Cells / virology
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HIV-1 / genetics*
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HeLa Cells
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Herpesvirus 1, Human / genetics*
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Human T-lymphotropic virus 1 / genetics*
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Humans
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RNA, Messenger / metabolism
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RNA, Viral / metabolism
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / physiology*
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Trans-Activators / genetics
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Trans-Activators / physiology*
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Transfection
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Viral Envelope Proteins / genetics*
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Viral Proteins / genetics
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Viral Proteins / physiology*
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rev Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, rev
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Gene Products, rex
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Gene Products, tax
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RNA, Messenger
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RNA, Viral
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RNA-Binding Proteins
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Trans-Activators
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US11 protein, herpesvirus
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Viral Envelope Proteins
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Viral Proteins
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rev Gene Products, Human Immunodeficiency Virus