In a study of 13 local and four reference strains of Haemophilus ducreyi all grew well on a selective medium consisting of Bacto proteose No 3 agar (Difco), soluble starch, IsoVitalex, human blood, and vancomycin. All the strains reduced nitrate, were alkaline-phosphatase-positive, and (with one exception) used glucose, fructose, and mannose, beta-lactamase was produced by 12 local strains. Erythromycin was the the most effective antibiotic tested, followed by streptomycin, co-trimoxazole, and spectinomycin.
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