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Human papillomavirus PCR direct sequencing study of cervical precancerous lesions in Quebec children
  1. Luc L Oligny1,
  2. Juan Carlos Feoli-Fonseca1,
  3. Pierre Brochu1,
  4. Pierre Simard1,
  5. Sarah Falconi2,
  6. Wagner V Yotov3
  1. 1Département de Pathologie, Hôpital Ste-Justine, Montréal, Québec, Canada
  2. 2Programme de Biologie Moléculaire, Faculté de Médecine, Université de Montréal, Montréal, Québec, Canada
  3. 3Département de Pathologie, Hôpital Ste-Justine, Montréal, Québec, Canada; Département de Pédiatrie, Université de Montréal; Département de Biochimie, Université de Montréal, Québec,
  1. Dr Wagner Yotov, Hôpital Ste-Justine, Local 4731, 3175 Côte Ste-Catherine, Montréal, Québec H3T 1C5, Canadayotovw{at}

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Editor,—Similarly to adult pathology, human papillomavirus (HPV) infection is the most common sexually transmitted disease in adolescent girls, whose prevalence is 16% according to one US study.1 However, little or no HPV sequencing data from paediatric specimens are available. We used our two tier polymerase chain reaction (PCR) direct sequencing (PCR-DS) approach2 to study cervical biopsies from 44 adolescent Quebec girls (14–17 years old). They originated from various social and ethnic groups, as well as geographically distinct areas of Quebec. Written informed consent about the use of the specimens was obtained from the ethics committee of this institution. All biopsies were analysed for histological changes and presence of HPV specific DNA. Most of them (n = 36) were diagnosed as cervical intraepithelial neoplasia (CIN), seven as inflammatory changes, and one as …

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