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Nucleic acid amplification tests have the ability to specifically amplify small quantities of DNA and hence have been used successfully in the diagnosis of STDs1, 2 An in-house polymerase chain reaction (PCR) method was developed and evaluated for the detection of Neisseria gonorrhoeae DNA in the urogenital specimens collected (with consent) from patients visiting an STD clinic in India.
The primers (forward primer 5'-CAACTATTCCCGATTGCGA-3' and reverse primer 5'-GTTATACAGCTTCGCCTGAA-3') amplify the 221–480 bp region of orf1 gene. Clinical isolates (n = 40) of N gonorrhoeae were recovered from urethral or cervical swabs by inoculation onto modified Thayer-Martin medium and identified by Gram stain, colony morphology, positive oxidase, and rapid carbohydrate utilisation test. For PCR the clinical samples (n …