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Four layer discontinuous gradient for HIV
  1. K Kakimoto,
  2. Y Ando,
  3. A Yoshioka
  1. International Medical Centre of Japan, Tokyo, Japan
  1. Correspondence to:
 K Kakimoto;
 kakimoto{at}sannet.ne.jp

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Artificial insemination using processed semen is a risk reduction option, if they want children, for serodiscordant couples in whom the man is HIV positive. The main aim of this study was to develop a single semen processing technique to reduce HIV transmission risks to HIV negative wives without infection and to obtain better quality sperm.

Methods

After ethics committee approval and written informed consent, normozoospermic semen was provided by two asymptomatic HIV carriers. Discontinuous four layer density gradient, whose fractions (Fr) were 1.065 (Fr 4), 1.085 (Fr …

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