Article Text

Download PDFPDF
Four layer discontinuous gradient for HIV
  1. K Kakimoto,
  2. Y Ando,
  3. A Yoshioka
  1. International Medical Centre of Japan, Tokyo, Japan
  1. Correspondence to:
 K Kakimoto;

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

Artificial insemination using processed semen is a risk reduction option, if they want children, for serodiscordant couples in whom the man is HIV positive. The main aim of this study was to develop a single semen processing technique to reduce HIV transmission risks to HIV negative wives without infection and to obtain better quality sperm.


After ethics committee approval and written informed consent, normozoospermic semen was provided by two asymptomatic HIV carriers. Discontinuous four layer density gradient, whose fractions (Fr) were 1.065 (Fr 4), 1.085 (Fr …

View Full Text