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Comparison of culture and different PCR assays for detection of Trichomonas vaginalis in self collected vaginal swab specimens
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  • Published on:
    Author's reply
    • Tania Crucitti, Scientific collaborator
    • Other Contributors:
      • Eddy Van Dyck

    Dear Editor

    The purpose of our study was to evaluate five different primer sets described in the literature for the amplification of Trichomonas vaginalis. We therefore used the same working conditions for the five primers sets, i.e. the same extraction method, thermocycler, reagents etc. It was not our aim to re-validate these primer sets.

    For all of the five primer sets we used the AmpliTaq Gold poly...

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    Conflict of Interest:
    None declared.
  • Published on:
    Taq Polymerase vs. Ampli Taq Gold Polymerase for Trichomonas vaginalis PCR detection.
    • Robert H. Gilman, Proffessor, Director of the Tropical Medicine Institute
    • Other Contributors:
      • Holger Mayta, Maritza Calderon

    Dear Editor

    The article by Crucitti et al.[1] evaluated five PCR techniques for Trichomonas vaginalis including the one published by our group (Mayta et al. [2]). The authors however did not follow the protocol we published and so got results that we consider to be erroneous.

    In our work we used simple Taq polymerase while Crucitti used Taq gold for this purpose. He did this without chang...

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    Conflict of Interest:
    None declared.