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Historical correlates of genital herpes simplex virus type 2 infection in men attending an STD clinic
  1. J M Sizemore, Jr1,
  2. F Lakeman2,
  3. R Whitley2,
  4. A Hughes1,
  5. E W Hook III1,3
  1. 1Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL, USA
  2. 2Division of Pediatric Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL, USA
  3. 3Jefferson County Department of Health, Birmingham, AL, USA
  1. Correspondence to:
 James M Sizemore Jr
 The University of Alabama at Birmingham 703 19th Street South, 242 Zeigler Research Building Birmingham, AL 35294–0007, USA;


Background/objectives: While nearly one in four Americans has antibodies to HSV-2, only one of 40 reports a history of genital herpes (GH). The goal of this study was to correlate questions designed to elicit a GH history with serological evidence of HSV-2 in male STD clinic attendees.

Methods: Consecutive males were enrolled in a study of the epidemiology of GH. Consenting men answered questionnaires regarding their histories of possible GH and underwent serological testing for HSV-1 and HSV-2. Association statistics between response to each question and HSV-2 serological status were calculated.

Results: Of 328 men enrolled, 148 (46%) had HSV-2 antibodies. 14 (4.3%) reported a history of GH when queried as part of a list of other STD (sensitivity (S) 0.08). 17 (5.2%) reported a history of GH when asked directly “Do you have genital herpes?” (S 0.09). 75 (21.1%) participants reported a history of a recurring genital sore, ulcer, or zipper cut (S 0.32). Overall, 64.2% of HSV-2 seropositive men answered “no” to all three questions. A “yes” response to any of the questions was only 36% sensitive for predicting HSV-2 infection.

Conclusion: Few HSV-2 infected men report either a history of GH or are aware that they are infected. Asking about a history of recurrent genital sores was a more sensitive historical marker of HSV-2 infection than asking about a history of “genital herpes.”

  • GH, genital herpes
  • HSV, herpes simplex virus
  • herpes simplex virus
  • genital herpes
  • men

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In the United States, one in four adults is infected with herpes simplex virus type 2 (HSV-2). Most, however, do not recognise this infection; in the National Health and Nutrition Examination Survey (NHANES) III, while one in 40 participants reported a history of genital herpes (GH), only one in 10 of those infected stated they had GH. Furthermore, only one in 30 black people, a population with substantially higher HSV-2 prevalence, reported a history of GH.1

A published study from Seattle suggests that three quarters of infected individuals who do not report a history of GH actually have unrecognised infection, not truly asymptomatic infection.2

To further address patient recognition of HSV-2 infections, we examined historical correlates of HSV-2 among men attending an STD clinic in the south eastern United States, a previously understudied population.


Study population

The study was conducted at the Jefferson County Department of Health STD Clinic in Birmingham, AL. Consecutive heterosexual men presenting to the clinic were approached for enrolment.

This study was reviewed and approved by the institutional review board for human subjects at the University of Alabama at Birmingham.

Study procedures

After providing written informed consent, participants were administered a questionnaire collecting demographic data, STD history, sexual partner history, and information on GH knowledge.

Included were three questions targeting a history of GH. Firstly, participants were asked if they had ever had GH among a list of STDs; secondly, in a different section assessing knowledge about herpes, subjects were asked, “Do you have genital herpes?” Finally, men were later queried, “Have you ever had a recurring genital sore, ulcer, or zipper cut on your penis, scrotum, or anus?”

Participants then underwent a physical examination. Urine specimens for cervical gonorrhoea and Chlamydia trachomatis were collected; serum was obtained for VDRL, HIV, and HSV serological testing. Participants then received care per clinic protocol.

Herpes laboratory procedures

Serological testing for HSV-1 and HSV-2 was conducted in the laboratory of RW and FL using Herpeselect-1 and Herpeselect-2 ELISA IgG (98% sensitivity, 96% specificity, Focus Technologies, Inc, Herndon, VA, USA).

Data management and statistical analysis

Questionnaire and laboratory data were entered into a Microsoft Access database with appropriate checks to ensure accuracy.

Analyses were conducted with SAS software (SAS Institute, Cary, NC, USA). Odds ratios were calculated using Fisher’s exact test.


Study population

Between February 2002 and September 2003, 328 men were enrolled. The majority were young (median age, 26 years) and black (90%). Reasons for clinic visit included symptoms (52.3%), check up (30.3%), STD contact (16.2%), and other (1.2%).

These men had a substantial duration of sexual experience (median 11 years) defined as their age of sexual debut (median 15 years) subtracted from their current age. Participants reported a median of one partner in the last 30 days and 16 lifetime partners.

At the study visit, 45% (148/328) of men were HSV-2 seropositive, while 62.2% were HSV-1 seropositive.

When asked if they had GH among a list of STDs, 4.3% (14/328) of the overall population and 8.1% (12/148) of HSV-2 seropositive men answered affirmatively (odds ratio (OR) 7.8, p = 0.0018). Similarly, 5.2% (17/328) of all participants and 9.5% (14/148) of those with HSV-2 infection answered “yes” when asked directly, “Do you have genital herpes?” (OR 6.2, p = 0.0015). Ten of the 21 participants responding “yes” to one of the questions answered “yes” to both questions. Finally, 23% (75/328) of all men and 32.4% (48/148) of HSV-2 infected men reported a history of previous recurrent genital ulcer, sore, or zipper cut (OR 2.7, p = 0.0002) (table 1). Sixty four per cent (n = 95) of HSV-2 seropositive men answered “no” to all three questions; a “yes” response to any of the three questions was 36% (53/148) sensitive for predicting HSV-2 infection. There was no difference in herpes historical accounts among those infected with HSV-2 when stratified by HSV-1 serological status (p = 0.35).

Table 1

 Comparison of genital herpes historical correlates among 328 male STD clinic attendees


The sensitivity of reporting a history of GH for predicting HSV-2 infection as measured by antibody is uniformly low in both general and high risk populations. When asked about a history of GH among a list of STD, our findings were similar to those reported from NHANES III. The sensitivity of a self reported history of GH in that study for the general population and for black people was 9.2% and 3.7%, respectively1; it was also noted that those who were HSV-2 seropositive only were three times more likely to report a history of GH (16.2%) than individuals seropositive for HSV-1 and HSV-2 (5.6%),3 suggesting that those co-infected with HSV-1 may have been less symptomatic. Similarly, in another study conducted among randomly selected attendees of primary care offices of six US cities, Leone et al report that one in four individuals were infected with HSV-2, though only 4% disclosed a history of GH; there, the sensitivity of reporting a GH history for predicting presence of HSV-2 antibody was 11.9%.4

Previous studies addressing self recognition of HSV-2 infection in high risk individuals are few. One study that examined self reported GH history in the context of screening was a secondary cross sectional analysis from Project Respect, a randomised trial of STD prevention programmes among STD clinic attendees. Of 4128 individuals attending five US STD clinics, 40.8% (1686/4128) were HSV-2 seropositive; among these only 11.7% reported a history of GH. Participants who were black, female, or HSV-1 seropositive were more likely to have unrecognised GH.5

Our findings regarding heterosexual high risk men confirm as well as add new insights to these published data. Firstly, genital HSV-2 infection was common and poorly recognised. However, when questioned using different queries to elicit a history of possible GH, this predominantly black STD clinic population recognised infection three times more commonly than reported in NHANES III. Asking about recurring genital lesions was a more sensitive historical marker for HSV-2 infection than asking directly about genital herpes, suggesting that a substantial proportion of these men recognise abnormalities but do not associate them with GH. Finally, while the proportion of those seropositive for HSV-1 infection was not different from those of NHANES III and Project Respect, we did not identify HSV-1 serostatus as a modifying factor in the recognition of genital HSV-2 infection as had been reported previously.

Several limitations of this study should be acknowledged and considered. Firstly, these data were collected from a single STD clinic in the south eastern United States, and, thus, may not be generalisable to other populations. None the less, we have described participants from the racial ethnic group (black people) in whom HSV-2 seroprevalence is highest, recruited from the region (southeastern United States) where infection is more common.1 Finally, a few responses from those who have genital HSV-1 disease could have been misclassified, though we have found little evidence of HSV-1 genital infection in this population.


Though HSV-2 is common, few men report a history of GH. Asking about a history of recurrent genital sores was a more sensitive marker of HSV-2 infection than asking about a history of GH, though most did not recognise the former fact as evidence of infection. Antibody screening and patient education about manifestations of genital herpes should be emphasised as important prevention tools against the spread of HSV-2 among high risk heterosexual males.


Special thanks to Marga Jones for assistance with data management.

 JS, protocol development, participant enrolment, manuscript preparation, revision; FL, herpes laboratory testing, manuscript review; RW, protocol development, herpes laboratory testing, manuscript review; AH, participant enrolment, manuscript review; EH, protocol development, non-herpes STD laboratory testing, manuscript review, revision.



  • Grant support: Supported by the UAB Sexually Transmitted Diseases Cooperative Research Center Grant 5 U19 AI38514-07, the UAB GCRC, RR00032.

  • No conflict of interest for any of the authors.

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