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Interview and volunteer counselling and testing procedures were conducted at a conveniently located HIV clinic, where blood was also drawn to test for HIV and other sexually transmitted diseases. Samples were first screened for HIV antibodies by ELISA, and then confirmed with a Western Blot Assay. Syphilis screening was performed using the Rapid Plasma Reagin Test and plasma specimens that were positive were confirmed by Treponema Pallidum Particle Agglutination (TPPA) Assay. Participants with a positive test result at any follow-up visit were referred to medical and social services and were asked to leave the study.
Funding This work was supported in part by National Natural Science Foundation of China (81001288); ‘National ST Major Project Foundation of China’ (No. 2012ZX10001-001 and No. 2011ZX10004-902).
Competing interests None.
Patient consent Obtained.
Provenance and peer review Not commissioned; internally peer reviewed.
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