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Influence of anaerobic conditions on vaginal microbiota recovery from bacterial vaginosis patients
  1. Tatiana B Cereija,
  2. Joana Castro,
  3. Patrícia Alves,
  4. Nuno Cerca
  1. Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, University of Minho, Braga, Portugal
  1. Correspondence to Dr Nuno Cerca, Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, University of Minho, Gualtar Campus, Braga 4710, Portugal; nunocerca{at}ceb.uminho.pt

https://doi.org/10.1136/sextrans-2013-051100

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    Bacterial vaginosis (BV) is one of the most common infections in women of reproductive age. Clinical studies have shown an association among BV and abnormal pregnancy, pelvic inflammatory disease and an increased risk of sexually transmitted infections, including HIV.1 This disorder was first described in 1914 by Curtis as a ‘white discharge’ syndrome2 and despite the decades of research we have only limited, and clearly not conclusive, evidence of microbial cause of BV, mechanism of disease and effective treatment.

    The development of molecular techniques such as Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing produced a clearer picture of the …

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    Footnotes

    • Contributors NC designed the experiment. TC, JC and PA have performed the growth isolation and identification of bacteria. All the authors performed data interpretation, drafted the article and approved the final version. Vaginal samples were obtained from volunteers that signed an informed consent form approved by Institutional Review Board (SECVS).

    • Funding Fundação para a Ciência e Tecnologia (FCT).

    • Competing interests None.

    • Ethics approval Institutional Review Board (SECVS).

    • Provenance and peer review Not commissioned; internally peer reviewed.