Article Text

Download PDFPDF
Short report
A new rapid molecular point-of-care assay for Trichomonas vaginalis: preliminary performance data
  1. David M Pearce1,
  2. David N Styles1,
  3. Justin P Hardick2,
  4. Charlotte A Gaydos2
  1. 1Atlas Genetics, Trowbridge, Wiltshire, UK
  2. 2Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
  1. Correspondence to Dr David M Pearce, Atlas Genetics, Derby Court, White Horse Business Park, Trowbridge, Wiltshire BA14 0XG, UK; david.pearce{at}


Objective Trichomonas vaginalis infection is the most prevalent treatable sexually transmitted infection (STI) in the world. An accurate point-of-care (PoC) molecular test would enable patients to be tested and treated for T vaginalis in a single visit to the genitourinary medicine clinic, community STI clinic, pharmacy or doctor's office. In this report, we describe a rapid prototype assay for T vaginalis designed for use in conjunction with the Atlas io PoC platform, and initial verification of its performance using 90 clinical samples.

Methods A rapid prototype T vaginalis assay was designed. The test, featuring novel electrochemical end-point detection, used a multi-copy region of the T vaginalis genome as the assay target. Ninety clinical vaginal swab samples were used to verify the performance of the prototype assay.

Results The assay demonstrated a sensitivity and specificity of 95.5% (42/44) and 95.7% (44/46), respectively, when tested using clinical samples. Assay inclusivity was demonstrated for a number of geographically diverse T vaginalis isolates, and the test showed no cross-reactivity with either human DNA or a panel of DNAs isolated from common cross-reactants.

Conclusions The sensitivity and specificity achieved using this prototype assay is comparable with that achieved for existing central laboratory nucleic acid amplification tests used for screening patients for T vaginalis.


Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.