Chlamydia trachomatis (CT) infection spreads in Europe and in USA, and tendencies analysis shows an increase in the epidemic since last 10 years. In France, in 2006, a national survey carried out by phone and using a home-based sampling showed that CT prevalence was the highest in young people, with 3.6% for women and 2.4% in men aged 18–24 yo. To face this problem, several countries have developed new strategies, mixing newer technologies and home-based self-sampling test. Inspired by evaluation of those dispositives, the French National Institute of Health Prevention and Education (INPES) decided to experiment the proposition of a free home-based self-sampling to screen this infection via internet in young people 18–24 yo. This study, named Chlamyweb, aims to compare CT screening rate from this intervention with traditional information system and screening centre. Study design was a random control trial, with a 1:2 randomization. Recruitment took place on an Internet information website on sexually transmitted infections, and support by web campaign from September 3 to October 14 2012. Home-based kits were composed by uriswab 3 sponges for men and dry vaginal swabs for women (Copan diagnostics). All samples were analysed by using the fully automated cobas 4800 (Roche diagnostics). Self-sampling was proposed to 5 531 people. Out of them, 47.3% accepted, with a higher rate in women (53.0%). A total of 1616 kits provided [1002 from women (63.8%) and 614 from men (58.8%)] was return to the French National Reference Center for chlamydial infections. The global prevalence was 6.8% (8.3% in women, 4.4% in men). Sexual behaviour and sociodemographic patient’s characteristics were collected and their analysis is under investigation. These preliminary results show that Internet testing reaches a population with a high prevalence of CT infection and appears to be acceptable to young people.