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P2.024 Evaluation of the Genital Microbiota in Men and Women Using an Automated System For Analysis of Microscopy Images of Wet and Stained Smears
  1. E Rybina,
  2. E Bulatova,
  3. Z Martikainen,
  4. S Zatsiorskaya,
  5. M Zaitseva,
  6. V Panteleyev
  1. A Savicheva, Eastern European Network for SexualReproductive Health, Ott Institute of Obstetrics and Gynecology Academy of Medical Sciences of Russian Federation, St Petersburg, Russian Federation


Background In Russia, microscopy of the genital microbiota in both women and men are performed at laboratory, not at physician’s office. Microscopy is a simple and cheap method, which, together with a clinical presentation, provides sufficient information for diagnosis of bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, cervicitis, urethritis. Improvement of microscopy diagnostic methods with the use of automated computerised system is important.

Methods Clinical samples were obtained from 100 men and 150 women of reproductive age. In total, 150 vaginal, cervical and female urethral samples, 73 male urethral samples and 17 prostatic samples were analysed using microscopy of Gram and methylene blue stained preparations. The presence of polymorphonuclear leukocytes, lactobacilli, “clue” cells, yeasts, trichomonads, gramme-negative diplococci was assessed. Vaginal samples were also assessed for bacterial vaginosis using the Nugent score. For analysis, image analyzer including a Biological Microscope MT5000 Series, Neiji Techno Co., Ltd (Japan), digital colour camera Progress CT3 and software UroGyn were used.

Results In 17.33%, 11.33% and 2% of the vaginal samples, “clue” cells, yeasts and trichomonads were visualised by microscopy. Signs of cervicitis were detected in 2% of the women, in none of the female samples signs of urethritis were seen. In men, signs of urethritis were revealed in 3.45%, and prostatitis - in 29.4% of the samples. In none of the samples, Gram-negative diplococci were detected. There was complete agreement between the results obtained using the image analyzer and those obtained using traditional light microscopy.

Conclusion An automated system for the analysis of images obtained by microscopy investigation of urogenital samples from women and men is easy to use, allows documentation of results and facilitates their interpretation.

  • Diagnosis
  • genital smear microscopy
  • women

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