Objectives Moderate alcohol consumption can impair host defence against viral infections. The objective of this cross-sectional analysis was to assess the association between alcohol intake and prevalent human papillomavirus (HPV) infection among US men enrolled in the HPV in Men (HIM) study using quantitative alcohol intake measured from a Food Frequency Questionnaire.
Methods The HIM study is a prospective, multinational study of the natural history of HPV infection. For this report, we restricted our analyses to men from the US cohort (N =1313). Samples from the corona of glans penis, penile shaft and scrotum were combined for HPV DNA testing. Self-reported alcohol intake was quantified by grams of alcohol intake per day. Multivariable prevalence ratios (mPRs) were used to assess the association between alcohol intake and HPV infections.
Results Prevalent infections were significantly higher among men in the highest quartile of alcohol intake and multivariable models revealed that the highest quartile of alcohol intake was associated with significantly increased risks for any (mPR=1.13; 95% CI 1.00 to 1.27) HPV types and oncogenic (mPR=1.35; 95% CI 1.08 to 1.68) HPV types. The fourth quartile of alcohol intake was associated with elevated risks for prevalent HPV infection across all strata of number of sexual partners and among never-smokers and current smokers, but not among former smokers.
Conclusions These results demonstrate that high intake of alcohol is associated with an increased risk for prevalent HPV infections among men. The biological role that alcohol plays in genital HPV infection remains understudied and limited epidemiological data exist, especially among men.
- EPIDEMIOLOGY (GENERAL)
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With more than six million new infections occurring annually in the USA,1 ,2 human papillomavirus (HPV) is one of the most common sexually transmitted infections. There are more than 120 different HPV types, of which 40 or more types are transmitted through sexual contact.3 In addition to the clinical endpoints HPV causes in men, including genital warts and various cancers, HPV is readily transmitted from person to person and is strongly associated with cancer risk in women.4–6 Although the majority of HPV infections are transient and do not result in disease, the failure to develop an immune response to control an infection results in viral persistence and, in the case of the oncogenic HPV types, an increased risk of progression to cancer.7
Alcohol consumption is a potent modulator of immune function, which can lead to immune deficiency and increased susceptibility to various chronic and infectious diseases.8–11 Chronic alcohol abuse and acute and moderate alcohol consumption can adversely affect the immune system.9 ,11–13 Pathogen response is divided into two phases: the first phase is an inflammatory reaction, which provides protection against the immediate effects of the infection, and the second phase involves the development of immunity to the pathogen. Alcohol consumption can interfere with both phases of the immune response.9 The consequences of alcohol-induced immunodysfunction include increased susceptibility to numerous infectious endpoints, including bacterial pneumonia, septicaemia, tuberculosis and hepatitis.10 ,12 ,13 Currently, there are few published data on the association between alcohol consumption and genital HPV infection among men. Revealing the association between a potential risk factor and prevalent HPV infections is an obligatory step prior to initiating longitudinal analyses of HPV infection endpoints. Thus, the objective of this analysis was to use alcohol consumption data from a Food Frequency Questionnaire (FFQ) to assess the association between alcohol intake and prevalent HPV infection among US men in the HPV in Men (HIM) study. To evaluate the potential effect modification, we also stratified the data by smoking status and lifetime number of sexual partners. Until now, this is one of the largest analyses exploring the association between alcohol intake and HPV infection.
Materials and methods
Study population and Risk Factor Questionnaire
The human-subjects’ committees from The University of South Florida (USF) approved all study procedures before study initiation (USF IRB# 102660). The HIM study is a prospective, multinational study of the natural history of HPV infection in men; a full description of cohort procedures, HPV prevalence and factors associated with prevalent infections has been published.14 ,15 For this report, we restricted our analysis to men from the US cohort because of potential regional and cultural differences in alcohol consumption (ie, types of alcohol, frequency of use and age of initiation) and behaviour. Men who provided informed consent had a clinical examination 2 weeks prior to the enrolment visit (N=1427) and every 6 months thereafter. Only men who returned for the enrolment visit (N=1313) from 2005 through 2006 were included in this report.
An extensive Sexual History and Health Questionnaire, which required approximately 15 min to complete, was administered at the enrolment visit to assess sociodemographic information and risk factors. Using the US Centers for Disease Control definition,16 never-smokers were defined as men who had smoked <100 cigarettes in their lifetime. Likewise,17 former smokers were defined as men who had smoked at least 100 cigarettes in their lifetime but quit smoking at least 1 year before the enrolment interview. Current smokers were defined as men who smoked at least 100 cigarettes in their lifetime and were currently smoking (or quit within the previous 12 months) at the time of the enrolment visit.
Arizona Food Frequency Questionnaire (AFFQ)
The Arizona Food Frequency Questionnaire (AFFQ) is a modification of the National Cancer Institute's Health Habits and History FFQ18 that consists of a semiquantitative 159-item questionnaire which asks respondents to report how often they usually consumed each particular item over the prior 12-month period. The AFFQ was completed by the men at the enrolment visit and required about 30 min to complete. The AFFQ contained questions on alcohol consumption including serving size and frequency of light beer, beer, wine and liquor. Serving size was subjectively defined as the average serving size compared with other men of the same age and classified as small, medium or large. Frequency of consumption was collected as 6+ times per day, 3–5 times per day, twice a day, once a day, 5–6 times a week, 2–4 times a week, once a week, 1–3 times a month and rarely/never. The FFQ analysis programme quantified alcohol intake by grams of alcohol intake per day and per cent calories from alcohol per day. All findings were consistent when alcohol intake was evaluated according to per cent calories from alcohol per day. In this report, we present results for grams of alcohol intake per day.
Sample collection, DNA extraction and HPV genotyping
Details of sample collection, DNA extraction and HPV genotyping have been published elsewhere.14 ,15 Briefly, three separate specimens were obtained from the corona of glans penis, penile shaft and scrotum, placed into 450 µL of Specimen Transport Medium and then combined into one sample before DNA extraction. The extracted DNA samples were tested for the presence of HPV types by amplification with the PGMY09/11 L1 consensus primer system19 ,20 and HPV genotyping was performed with the Linear Array method on all samples irrespective of the HPV PCR result (Roche Molecular Diagnostics, Alameda, California, USA). Only samples that tested positive for β-globin (99% at enrolment) were judged to be adequate and included in the analysis.
Four HPV categories were assessed in this analysis (ie, ‘any HPV’, ‘oncogenic HPV’, ‘non-oncogenic HPV’ and ‘quadrivalent vaccine types’). A participant was considered positive for ‘any HPV’ if he tested HPV positive by PCR or tested positive for at least one genotype. The ‘oncogenic HPV’ category included men who were positive for at least one of the 13 oncogenic types tested (HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 66) and included men infected with both oncogenic and non-oncogenic types. ‘Non-oncogenic HPV’ infections included single or multiple infections with only non-oncogenic HPV types (6, 11, 26, 40, 42, 53, 54, 55, 61, 62, 64, 67, 68–73, 81–84, IS39 and CP6108). The ‘quadrivalent vaccine types’ included men with one or more prevalent infections of HPV 6, 11, 16 or 18.
All statistical analyses were performed using R V.2.14 (R Project for Statistical Computing, http://www.r-project.org) and SAS V.9.3 (Cary, North Carolina, USA). Alcohol intake was categorised by the quartile intake values among HPV-negative men. Sociodemographic and sexual behavioural cohort characteristics across quartiles of alcohol intake were compared by using Fisher’s exact test. The Wilcoxon rank-sum test was used to test for differences in the median alcohol intake and the Pearson's χ2 test was used to test for differences in the distribution of HPV positivity across quartiles of alcohol intake. Multivariable Poisson regression (PROC GENMOD) was used to generate prevalence ratios (PRs) and 95% CIs.
Alcohol consumption was categorised according to quartiles of grams (g) of alcohol intake per day among HPV-negative men: Q1 <0.10 g/day; Q2 ≥0.10 to <3.13 g/day; Q3 ≥3.13 to <9.91 g/day and Q4 ≥9.91 g/day (table 1). The mean (SD) of grams of alcohol intake per day was 0.02 g/day (0.02), 1.69 g/day (0.84), 5.88 g/day (2.02) and 35.9 g/day (39.8) in quartiles 1–4, respectively. Statistically significant differences were observed for the distribution of study population characteristics by quartiles of alcohol intake. Men who consumed higher levels of alcohol (quartiles 3 and 4) were younger, current smokers, White, reported more female sexual partners and were more likely to be circumcised (table 1).
Compared with HPV-negative men, the median intake of alcohol was significantly higher among HPV-positive men (table 2). The median alcohol intake per day among HPV-negative men was 3.13 g (IQR 0.1–9.9) compared with men who were positive for any HPV types (median=4.52 g; IQR 0.6–15.5; p<0.001), the oncogenic HPV types (median=5.23 g; IQR 1.1–18.3; p<0.001), the non-oncogenic HPV types (median=5.29 g; IQR 0.6–17.5; p=0.006) and the quadrivalent vaccine HPV types (median 6.31 g; IQR 1.2–19.4; p<0.001). When HPV prevalence was analysed by quartiles of alcohol intake (table 2), we noted a significantly higher prevalence of HPV among men in the highest quartile of alcohol consumption. Across the four quartiles of alcohol intake, the prevalence was 56.7%, 56.2%, 57.9% and 68.9% for any HPV types (p<0.001); 22.8%, 24.7%, 27.0% and 35.2% for oncogenic HPV types (p<0.001); 16.1%, 12.0%, 15.5% and 19.5% for non-oncogenic HPV types (p=0.002) and 11.7%, 12.0%, 15.1% and 19.5% for the quadrivalent vaccine HPV types (p<0.001).
In table 3, we present the multivariable prevalence ratios (mPRs) for the association between alcohol intake and HPV infection, adjusting for potential confounders including age, race, smoking status, ethnicity, circumcision, total number of female partners in the last 3 months and total number of female sexual partners. Overall, the highest quartile (Q4) of alcohol intake compared with the lowest quartile (Q1) was significantly associated with an increased risk for any HPV types (mPR=1.12; 95% CI 1.03 to 1.27) and oncogenic HPV types (mPR=1.35; 95% CI 1.08 to 1.68), and a borderline significant increased risk for the quadrivalent vaccine HPV types (mPR=1.47; 95% CI 0.98 to 2.23). To increase statistical power, we also assessed alcohol intake by combining the first three quartiles of intake into a new referent group. As evident in table 2, HPV prevalence was similar for the first three quartiles (table 2) and the overall point estimates (table 3) for the first three quartiles clustered around 1.00. Generally, the point estimates for Q4 were similar, but the CIs were narrower. We did not adjust for sex with male partners since over 94% of the men in this analysis reported having zero male partners and there was no difference (p=0.739) across quartiles of alcohol intake as noted in table 1. We performed an exploratory analysis that included number of male sexual partners in the model and also restricted to men who reported zero male sexual partners; however, there was no appreciable difference in the point estimates (data not shown).
We also stratified the analyses by smoking status. Significant associations were observed for high intake (Q4) and HPV prevalence among never-smokers for any HPV types (PR=1.22; 95% CI 1.03 to 1.44) and oncogenic HPV types (PR=1.48; 95% CI 1.09 to 2.02), and borderline significant associations for the non-oncogenic HPV types and quadrivalent vaccine HPV types. There were no statistically significant associations among former smokers. We noted borderline significant associations among current smokers for high alcohol intake (Q4) compared with the grouped referent (Q1–Q3) for any HPV infections (mPR=1.14; 95% CI 0.95 to 1.37) and oncogenic HPV infections (HR=1.26; 95% CI 0.98 to 1.70).
We also stratified the association between alcohol intake and HPV infection by lifetime number of sexual partners (0–1, 2–9 and ≥10). For these analyses, we used the grouped referent (Q1–Q3) and found that high alcohol intake (Q4) was associated with relatively modest elevated point estimates across all strata of number of sexual partners (table 4).
Assessing the impact of a potential risk factor and prevalent HPV infections is an important step prior to initiating longitudinal analyses. Thus, this study sought to assess the association between self-reported alcohol intake and prevalent HPV infections among US men. Our analyses revealed that prevalent infections were significantly higher among men in the highest quartile of alcohol intake and multivariable analyses, adjusting for potential confounders, including sexual behaviour and smoking, revealed that the highest quartile of alcohol intake was associated with an increased risk of prevalent genital HPV infection. Furthermore, we found no evidence of confounding by sexual behaviour and smoking following stratification by these risk factors.
The association between alcohol consumption and HPV-related endpoints has been reported in other study populations. A cross-sectional study of men in the Danish Army reported that alcohol intake was associated with having multiple HPV types.21 A prospective study of the natural history of cervicovaginal papillomavirus infection in women22 found an elevated risk (relative risk=2.0; 95% CI 1.2 to 3.1) of incident HPV infection associated with high alcohol consumption. A cross-sectional study that assessed sexual practices and cervical HPV infection among college women23 reported alcohol use was significantly more frequent among women who were HPV DNA positive. A case–control study of both sexes from four clinics in Washington state24 reported that four alcoholic drinks/week was associated with nearly a twofold increased risk of genital warts (95% CI 1.0 to 3.6) and five or more alcoholic drinks/week revealed a 2.4-fold increased risk (95% CI 1.2 to 5.1). Conversely, two studies found no relationship between alcohol intake and HPV endpoints. A cross-sectional analysis of women seeking contraceptive advice in three Swedish clinics reported that recent use of alcohol was not associated with cervical HPV infection after adjustment for sexual/behavioural factors.25 In a separate study among high-risk HPV-positive women, alcohol intake was not associated with risk of high-grade squamous intraepithelial lesions.26 In spite of some inconsistencies in the literature, evidence suggests a modest association between alcohol consumption and prevalent HPV infection.
Previous studies in both men and women have shown that cigarette smoking is associated with HPV prevalence,27 ,28 incidence29 and persistence.30 We found significant point estimates for the association between alcohol consumption and HPV infection among never-smokers and borderline significant associations among current smokers. The observed effects among never-smokers are novel and of potential public health importance as there are few risk factors for HPV infection among never-smokers.
It is plausible that the association between higher alcohol intake and HPV infection could be due to increased sexual disinhibition and promiscuous sexual behaviour. To account for potential confounding, we adjusted for sexual activity in the multivariable models and we stratified by lifetime number of sexual partners to reveal potential effect modification by sexual activity. Interestingly, the stratified analyses demonstrated that high alcohol intake was generally associated with a modest increased risk of HPV risk infection regardless of the number of sexual partners. If increased sexual behaviour is solely responsible for our findings, we would have expected to see no association between high alcohol intake and HPV after adjustment and elevated effects only in the highest sexual activity strata. Because differences in sexual behaviour by alcohol intake do not appear to explain our findings, the observed associations could be due to other factors such as the systemic effects of alcohol on immune function. The immune system serves as a defence against infections, and alcohol consumption is a potent modulator of immune function.8–11 Studies in laboratory animals and in humans have demonstrated that acute and moderate alcohol consumption can transiently impair host defence against viral infections.9 Although the clinical implications of such a transient immunodepression are not completely understood, the epidemiological evidence adds insight into the putative consequences of alcohol consumption on HPV susceptibility.
There are both strengths and limitations in the present analyses. Because of the potential to report socially desirable responses, self-reported data have inherent biases that could lead to under-reporting of alcohol consumption, tobacco use and number of sex partners.31 ,32 Additionally, FFQs are subject to random and systematic error,33 and differential misclassification of dietary intake is a concern if a study participant was aware of his/her disease status. However, differential misclassification would not impact the present findings as men were not aware of their HPV status during the administration of the Risk Factor Questionnaire and FFQ. Also, FFQs cannot estimate intake from the remote past and have been shown to introduce biased associations,34 so we attempted to reduce potential measurement errors attributable to recall bias by assessing intake during the year prior to enrolment into the study. We acknowledge that we cannot account for bias due to unmeasured or unknown confounding. Although we accounted for potential confounding by adjusting for self-reported sexual behaviour and stratifying by smoking status and lifetime number of sexual partners, residual confounding may still exist which could potentially inflate the observed point estimates. We also acknowledge that the US men in the HIM cohort may not be a representative of the general male population of the USA, which may limit the generalisability of our findings.
Overall, the results from these analyses demonstrated that high intake of alcohol is associated with an increased risk for prevalent HPV infections. Although these results cannot be considered causal and should be interpreted with caution, our findings do provide additional support to current public health messaging regarding the importance of moderate alcohol consumption, smoking cessation and safe sex practices. The biological mechanisms underlying the association between alcohol consumption and genital HPV infection remains understudied and limited epidemiological data exist, especially among men. Additional research is needed to replicate the current findings before clinical interventions can be recommended. Nonetheless, these data are important since there is limited information on the association between alcohol consumption and genital HPV infection in men and future longitudinal analyses will be needed to assess whether alcohol consumption is associated with HPV acquisition and clearance.
This analysis revealed that the highest quartile of alcohol consumption is associated with an increased risk for prevalent human papillomavirus (HPV) infections.
We found no evidence of confounding by sexual behaviour and smoking following multivariable adjustment and stratification by these covariates.
High alcohol intake was also associated with increased risk of HPV infection among never-smokers and current smokers.
The observed effects among never-smokers are novel and of potential public health importance as there are few risk factors for HPV infection among never-smokers.
Review history and Supplementary material
Handling editor Jackie A Cassell
Acknowledgements The authors thank the following staff members for their dedication in recruiting, examining and maintaining data on cohort participants, as well as conducting HPV DNA laboratory analyses: Kathy Eyring, CCRP; Christine Gage, ARNP; Nadia Lambermont, ARNP; Kim Isaacs, BA; Andrea M. Bobanic, BA; Kayoko Kennedy, BA and the Tissue Core staff of the Moffitt Cancer Center for their help in managing biological samples from the US site.
Contributors MBS, KME and ARG collaborated in the writing of this manuscript. MRP, BNT and MEA were involved in management of the cohort, data management and assisted in editing the manuscript. ARG was involved in project design and management as the principal investigator of the grant funding the project. MBS, ZJT, BNT and AN were responsible for data analysis. MBS, KME and ARG were responsible for data interpretation.
Funding This work was supported by the National Cancer Institute at the National Institutes of Health Grant (CA R01CA098803).
Transparency declaration The MBS (the manuscript's guarantor) affirms that this manuscript is an honest, accurate and transparent account of the study being reported; that no important aspects of the study have been omitted and that any discrepancies from the study as planned (and, if relevant, registered) have been explained.
Competing interests None.
Ethics approval University of South Florida.
Provenance and peer review Not commissioned; externally peer reviewed.
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