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P089 Detection of Neisseria gonorrhoeae bacterial loads in the pharynx and saliva among men who have sex with men
  1. Eric Chow1,2,
  2. Sepehr Tabrizi3,4,
  3. Samuel Phillips3,5,
  4. David Lee1,
  5. Catriona Bradshaw1,2,
  6. Marcus Chen1,2,
  7. Christopher Fairley1,2
  1. 1Melbourne Sexual Health Centre, Alfred Health, Melbourne, VIC, Australia
  2. 2Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, VIC, Australia
  3. 3Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia
  4. 4Department of Microbiology and Infectious Diseases, The Royal Women’s Hospital, Parkville, VIC, Australia
  5. 5Department of Obstetrics and Gynaecology, University of Melbourne, Parkville, VIC, Australia
  6. 6Murdoch Childrens Research Institute, Parkville, VIC, Australia


Background/introduction Studies have shown that N. gonorrhoeae can be cultured in human saliva among individuals with pharyngeal gonorrhoea. As saliva could potentially act as a carrier for gonorrhoea transmission during sex, the bacterial load of N. gonorrhoeae in saliva may influence the transmissibility of gonorrhoea.

Aim(s)/objectives To quantify the gonococcal bacterial load in the pharynx and saliva among men who have sex with men (MSM) with untreated pharyngeal gonorrhoea.

Methods MSM who tested positive for pharyngeal gonorrhoea by culture were recalled for antibiotic treatment within 14 days between October 2014 and March 2015. The gonococcal bacterial load was estimated using real-time quantitative PCR (qPCR) by interpolating against a standard curve generated with known gonococcal DNA copy numbers. The median of gonococcal bacterial load in the pharynx and saliva was calculated and compared between culture positivity using Mann-Whitney U test.

Results A total of 33 men were included in this study. At the time of treatment, the median gonococcal bacterial load in the pharynx was similar in men who were culture-positive (2.5 × 105 copies/swab) and culture-negative (2.9 × 104 copies/swab) (p = 0.166), and similar in the saliva in culture-positive: 2.2 × 105 copies/ml compared to culture-negative: 2.7 × 105 copies/ml samples (p = 0.499).

Discussion/conclusion The gonococcal bacterial loads were similar between saliva and the pharynx and not influenced by culture status. Saliva could be important in the transmission of gonorrhoea such as oral-anal sex and saliva use as a lubricant for anal sex.

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