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P1.55 Detection of genital mycoplasmas in women visiting the infertility clinic of an academic hospital, pretoria, south africa
  1. Thabang C Duba1,
  2. Remco Ph Peters2,
  3. Marthie M Ehlers3,
  4. Noëlle Pruis1,
  5. Sweetness M Majola1,
  6. Marleen Kock3
  1. 1University of Pretoria, Pretoria, South African Republic
  2. 2University of Pretoria/Anova Health Institute, Pretoria, South African Republic
  3. 3University of Pretora/National Health Laboratory Service, Pretoria, South African Republic


Introduction Sexually transmitted infections (STIs) continue to be a significant public health problem with a high burden in women of reproductive age. Rates of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis are frequently tested for and rates of infection are generally high in African settings, but the prevalence of other genital STIs is largely unknown. The aim of this study was to determine the prevalence of genital mycoplasmas (Mycoplasma genitalium, M. hominis, Ureaplasma parvum and U. urealyticum) in women visiting the infertility clinic of a tertiary academic hospital in South Africa.

Methods In this pilot evaluation self-collected vaginal swabs were obtained from 51 women visiting the infertility clinic. The genomic DNA was extracted from the swabs using the ZR Fungal/Bacterial DNA Miniprep (Thermo Scientific, USA) and analysed using the Anyplex II STI-7 (Seegene, Korea) real-time PCR assay for the simultaneous detection and identification of seven STIs including the four mycoplasma species.

Results The real-time PCR assay detected the following genital mycoplasmas and co-infections in the 51 women: U. parvum [55% (28/51)], M. hominis [20% (10/51)] and U. urealyticum [16% (8/51)]; none of the specimens tested positive for M. genitalium. Among the nine patients where mixed infections were observed, M. hominis and Ureaplasma spp. were frequently detected together [67% (6/9)]. In addition to the mycoplasmas, one woman tested positive for C. trachomatis; N. gonorrhoeae and T. vaginalis were not detected.

Conclusion This pilot study demonstrated an unexpectedly high rate of genital mycoplasma infections among women visiting an infertility clinic. The burden of genital mycoplasma infection is largely unknown and warrants further investigation, in particular with regards to the prevalence and clinical significance in different population groups.

Support: Anyplex II STI-7 kits provided by Seegene, Korea

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