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Stability of Chlamydia trachomatis RNA after long-term biobank storage
  1. Olivia Messina Rose1,
  2. David N Fredricks2,
  3. Olusegun O Soge3,
  4. Fouzia Farooq1,
  5. Gong Tang4,
  6. Roberta Ness5,
  7. Catherine L Haggerty1
  1. 1 Department of Epidemiology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
  2. 2 Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
  3. 3 Center for AIDS & STD, University of Washington, Seattle, Washington, USA
  4. 4 Department of Biostatistics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
  5. 5 School of Public Health, University of Texas, Houston, Texas, USA
  1. Correspondence to Dr Catherine L Haggerty, Department of Epidemiology, University of Pittsburgh, Pittsburgh, PA 15261, USA; haggerty{at}

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Archived vaginal samples are frequently used in ancillary research projects, yet few studies have validated their use for nucleic acid amplification analysis after long-term storage. This study sought to examine the suitability of vaginal swab samples for Chlamydia trachomatis transcription-mediated amplification analysis after up to 20 years of biobank storage.

Replication testing was conducted using a subset of vaginal swab samples originally obtained between May 1999 and June 2001 using …

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  • Handling editor Anna Maria Geretti

  • Contributors All authors have seen and approved the manuscript, have contributed significantly to the work and do not have conflicts of interest to report.

  • Funding 1R56AI139189-01, National Institutes of Health.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; internally peer reviewed.