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P387 Assessment of the performance of WHO prequalified HIV rapid tests and HCV rapid technology on Dried blood spot eluats
  1. Hicham Oumzil1,
  2. Lavinia Dorothea1,
  3. Imane Belbacha1,
  4. Ouafae Bennani1,
  5. Rajae Mengad1,
  6. Nezha Elomari1,
  7. Siham Amghar1,
  8. Azzedine Ibrahimi2,
  9. Elmir Elharti1
  1. 1Institut National d’Hygiène, Virology Department, Rabat, Morocco
  2. 2School Medecine, Rabat, Morocco


Background Rapid testing is a new technological alternative in the diagnostics of HIV and HCV. In view of their intrinsic performance, they can be used on several specimens: whole blood, serum; and plasmaThe present study aims to evaluate the performance of HIV and HCV rapid tests on Dried Blood Spots (DBS) eluates.

Methods Using a Respondent Driven Sampling protocol, 492 dried blood samples were collected from intravenous drug users. The serostatus of the samples towards HIV and HCV was determined by electrochemiluminescence (Cobase411 - Roche, USA) taken as the gold standard. HIV-positive cases were confirmed by Western Blot (HIVBlot 2.2, MPDiagnostics). The rapid tests evaluated are based on immunochromatography, are WHO prequalified: KHB Colloidal HIV 1 + 2 (KHB, China), SD Bioline HIV 1 + 2 (SDBionline, Korea) and UniGold HIV 1 + 2 (Unigold, Ireland). For HCV, these are: KHBColloidal HCV (KHB, China), INFO (Turklab, Turkie); and ABON (Abon, China).

Results From the HIV rapid tests assessment showed that SDBioline highlighted a specificity of 96.28% and a sensitivity of 70.97%, whereas KHB showed a sensitivity of 87.1% and a specificity of 98.51%. UniGold had the highest specificity (100%) and the lowest sensitivity (45.1%). In comparison with the HIV rapid tests, the HCV tests had very low specificities: 18.52% for KHB; 37.04% for INFO and 47.62% for ABON. On the other hand, sensitivity for all HCV rapid tests was 100%.

Conclusion To sum up, HIV and HCV rapid tests were shown to be able to detect anti-HIV and anti-HCV antibodies in DBS eluates. However Based on the overall results of the HCV rapid tests, it is recommended that DBS specimens eluate should not be used in HCV serological Tests. This indicates that there is a great need for further studies to argue the use rapid tests for serological screening of DBS eluatsts.

Disclosure No significant relationships.

  • Viral hepatitis
  • Co-infections
  • diagnosis

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