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P461 Bacterial load of chlamydia in the oropharynx and saliva among gay and bisexual men with untreated oropharyngeal chlamydia
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  1. Tiffany Phillips1,
  2. Christopher Fairley1,
  3. Kate Maddaford1,
  4. Jennifer Danielewski2,
  5. Jane Hocking3,
  6. David Lee1,
  7. Deborah Williamson4,
  8. Gerald Murray2,
  9. Fabian Kong3,
  10. Catriona Bradshaw1,
  11. Marcus Chen1,
  12. Benjamin Howden4,
  13. Eric Chow1
  1. 1Alfred Health, Melbourne Sexual Health Centre, Carlton, Australia
  2. 2The Royal Women’s Hospital, Centre for Women’s Infectious Disease Research, Parkville, Australia
  3. 3University of Melbourne, Melbourne School of Population and Global Health, Parkville, Australia
  4. 4The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, Microbiological Diagnostic Unit Public Health Laboratory, Parkville, Australia

Abstract

Background Previous studies have found that saliva can carry infectious gonorrhoea, which has led to the hypothesis that saliva could play an important role in gonorrhoea transmission. However, no study has examined the role of saliva in chlamydia transmission. The aim of this study was to determine whether Chlamydia trachomatis could be detected in saliva and to determine if the infection is specific to an anatomical site; oropharynx or tonsils.

Methods Men who have sex with men (MSM) who tested positive for oropharyngeal chlamydia at Melbourne Sexual Health Centre, who had no antibiotics in the past 4 weeks, and returned for treatment within 14 days between August 2017 and August 2018 were invited to participate. On the day of treatment, throat swabs were taken by clinicians at the tonsillar fossae and another at the posterior oropharynx. A saliva sample was also collected. All samples were tested for Chlamydia by nucleic acid amplification tests. The sample adequacy and bacterial load of Chlamydia trachomatis were assessed by quantitative PCR.

Results Forty-two MSM were included with a median age of 28 (Interquartile range [IQR]:25–33). The majority of men (76.2%; n=32) tested positive at both the tonsils and the oropharynx, followed by 9.5% (n=4) positive at the oropharynx only, and 4.8% (n=2) positive at the tonsils only. Chlamydia was detected in saliva in two-thirds of men (68.0%; n=29). The median bacterial load of chlamydia was 446 copies/ml (IQR: 204-1390 copies/ml) in saliva, 1230 copies/ml (IQR: 538–18200 copies/ml) from the tonsils and1660 copies/ml (IQR: 456–22400 copies/ml) at the oropharynx. The chlamydia loads did not differ between the tonsils and the oropharynx (p=0.865).

Conclusion Chlamydia can be detected in saliva in most of oropharyngeal chlamydia cases among MSM. Sampling both the tonsils and oropharynx is important for optimal detection of oropharyngeal chlamydia.

Disclosure No significant relationships.

  • chlamydia

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