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P670 Internationally disseminated ceftriaxone-resistant neisseria gonorrhoeae strain found in china
  1. Shaochun Chen,
  2. Yueping Yin
  1. National Center for STD Control, China CDC Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical College, Reference Lab, Nanjing, China

Abstract

Background Ceftriaxone has been used to treat gonorrhea in China for more than one decade, but an increasing level of decreased susceptibility or clinical resistance to ceftriaxone has been found. Moreover, the international spread ceftriaxone-resistant clones has been recognized as a threat to effective control of gonorrhea. We now describe an imported ceftriaxone-resistant N. gonorrhoeae strain isolated in China, 2016.

Methods The isolate was collected in 2016. The antimicrobial susceptibility to ceftriaxone (CRO), cefixime (CFM), azithromycin (AZM), spectinomycin (SPT) and ciprofloxacin (CIP) was determined using the agar dilution method in reference lab at National Center for STD Control. A combination of molecular epidemiological methods including N. gonorrhoeae multiantigen sequence typing (NG-MAST), multi-locus sequence typing (MLST) and N. gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR) was used to determine characteristics and resistant determinants of this isolate.

Results The strain was resistant to CRO (MIC 0.5 mg/L), CFM (MIC 1 mg/L), TET (4 mg/L) and CIP (≥32 mg/L), but susceptible to AZM (0.25 mg/L) and SPT (16 mg/L). The MLST type was ST1903, and NG-MAST type was ST3435. The NG-STAR type was ST233, which contains a type 60 mosaic penA allele, –35A Del in the mtrR promoter, G120K-A121D in PorB, L421P in PonA, S91F-D95A in GyrA, S87R in ParC, and wild-type 23srRNA.

Conclusion We identified a ceftriaxone-resistant N. gonorrhoeae strain which has sustainably transmitted worldwide for more than 3 years. The epidemiological and molecular typing data drew an integral transmission chain of this clone from Japan to China, and then disseminated globally. These findings indicate an imported risk of resistant clones in China and also call for an enhanced global gonococcal antimicrobial surveillance to track the emergence and dissemination of resistant strains for timely control the spread.

Disclosure No significant relationships.

  • Neisseria gonorrhoeae
  • antimicrobial resistance
  • trends

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