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P833 HSV-2 serostatus and HPV incidence, persistence, and precancerous lesions in a cohort of HPV-vaccinated women living with HIV
  1. Elisabeth Mcclymont1,
  2. François Coutlée2,
  3. Marette Lee3,
  4. Arianne Albert4,
  5. Sharon Walmsley5,
  6. Nancy Lipsky4,
  7. Gina Ogilvie6,
  8. Darrell Tan7,
  9. Deborah Money6
  1. 1University of British Columbia, Obstetrics and Gynecology, Vancouver, Canada
  2. 2l’Université de Montréal, Microbiologie Médicale et Infectiologie, Montreal, Canada
  3. 3BC Cancer, Vancouver, Canada
  4. 4Women’s Health Research Institute, Vancouver, Canada
  5. 5University Health Network, Toronto, Canada
  6. 6University of British Columbia, Vancouver, Canada
  7. 7St. Michael’s Hospital, Toronto, Canada


Background In understanding HPV oncogenesis, several co-factors have been proposed including co-infection with HSV-2. We assessed the relationship between HSV-2 serostatus and HPV-related outcomes in a cohort of quadrivalent HPV-vaccinated women living with HIV (WLWH).

Methods In this multi-site study of immunogenicity and efficacy of the qHPV vaccine in WLWH, three doses of qHPV vaccine were offered. Visits were at months -3, 0, 2, 6, 12, 18, 24, and annually thereafter. Participants provided clinical data and cervico-vaginal swabs for HPV DNA detection (Linear array assay) at each visit; baseline serum was tested for HSV-2 type-specific serology (Focus EIA). We used non-parametric statistics to compare the HPV-related outcomes (including 37 high and low-risk HPV types) according to HSV-2 serostatus.

Results 151 women aged ≥16 provided baseline serum samples for HSV-2 testing. The predominant regions of origin were Canada (51%) and Africa (30%). At baseline, median age was 39 years (IQR: 34–45), median CD4 count was 500 cells/mm3 (IQR: 382–692), and 70% had an HIV viral load <50 copies/mL. Baseline seroprevalence of HSV-2 was 76.2%, and median years of follow-up was similar for HSV-2 positive (6, IQR: 5.0–7.8) and negative (6, IQR: 5.2–7.9) participants. HSV-2 positivity was significantly associated with increased age. HSV-2 seropositive and seronegative participants had similar frequencies of HPV persistence (86/115 vs 27/36, p=1), clearance of incident HPV infections (88/115 vs 26/36, p=0.8), number of HPV types detected during the study (4.5 vs 5.7, p=0.1), HSIL cytology during the study (11/115 vs 2/36, p=0.7), and CIN2+ histology ever (15/115 vs 5/36, p=1). Results were similar in sensitivity analyses in which HSV-2 seropositivity was defined as an index value ≥3.5.

Conclusion HSV-2 seropositivity was common in this cohort of WLWH in Canada, but was not associated with multiple measures of HPV incidence, persistence, and precancerous lesions.

Disclosure No significant relationships.

  • HPV

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