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  • Improved sensitivity from pooled urine, pharyngeal and rectal specimens when using a molecular assay for the detection of chlamydia and gonorrhoea near point of care

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Research letter
Improved sensitivity from pooled urine, pharyngeal and rectal specimens when using a molecular assay for the detection of chlamydia and gonorrhoea near point of care
  1. Judith Ann Dean1,
  2. Sara Fiona Elizabeth Bell1,
  3. Luke Coffey2,
  4. Joseph Debattista3,
  5. Steven Badman4,
  6. Andrew M Redmond2,5,
  7. David M Whiley6,7,
  8. Jime Lemoire2,
  9. Owain David Williams1,
  10. Chris Howard2,
  11. Charles F Gilks1
  1. 1 School of Public Health, Faculty of Medicine, The University of Queensland, Herston, Queensland, Australia
  2. 2 RAPID, Queensland Positive People, Brisbane, Queensland, Australia
  3. 3 Metro North Public Health Unit, Metro North Hospital and Health Service, Windsor, Queensland, Australia
  4. 4 The Kirby Insitute, University of New South Wales, Sydney, New South Wales, Australia
  5. 5 Royal Brisbane and Women's Hospital, Metro North Hospital and Health Service, Herston, Queensland, Australia
  6. 6 Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia
  7. 7 Pathology Queensland, Queensland Health, Herston, Queensland, Australia
  1. Correspondence to Dr Judith Ann Dean, School of Public Health, Faculty of Medicine, The University of Queensland, Herston, QLD 4006, Australia; j.dean4{at}uq.edu.au

https://doi.org/10.1136/sextrans-2020-054835

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    For populations at risk of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG), regular testing of multiple anatomical sites is recommended. Pooling methods that combine one patient’s anatomical site specimens can reduce cost and workflow burdens.1 2 The feasibility of such methods within a community setting is well established3 4; however, sensitivities of pooling are inferior to methodologies that test anatomical sites separately.1 5 Our group sought to improve pooling performance near the point of care within a peer-led, community-based STI testing service.

    Adapting a protocol described by Speers et al,5 we previously reported3 sensitivities of 90% (95% CI 77.4% to 96.3%) and 89.7% (95% CI 74.8% to 96.7%) for the pooled detection of CT and NG, respectively, when using the Xpert CT/NG assay (Cepheid, Sunnyvale, California, USA). The Xpert CT/NG assay has not been listed by any regulatory …

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    Footnotes

    • Handling editor Anna Maria Geretti

    • Contributors JAD authored the manuscript with SFEB, LC and JD. All authors contributed to methodology formation, data analysis and the final manuscript.

    • Funding This study was conducted with support and under the auspices of the Queensland Professorial Chair in blood-borne virus (BBV) and STI. Cepheid provided funding and in-kind support under an Independent Research Support Agreement for phase II of the study.

    • Competing interests DMW reports research funding from SpeeDx Pty Ltd.

    • Provenance and peer review Not commissioned; internally peer reviewed.