RT Journal Article
SR Electronic
T1 Cultivation of type 1 N. Gonorrhoeae in liquid media.
JF The British Journal of Venereal Diseases
JO Br J Vener Dis
FD BMJ Publishing Group Ltd
SP 382
OP 386
DO 10.1136/sti.51.6.382
VO 51
IS 6
A1 Chan, K
A1 Wiseman, G M
A1 Caird, J D
YR 1975
UL http://sti.bmj.com/content/51/6/382.abstract
AB The effect of CO2 concentration on the growth and colonial stability of Type 1 Neisseria gonorrhoeae has been investigated. Carbon dioxide at a concentration of 16 per cent in air above flasks incubated in a shaker was effective in supporting growth and 100 per cent colonial stability of Types 1, 1', 2, and 4. Lower CO2 tensions increased the generation time of the strains and were less effective in maintaining the stability of virulent variants. Of several liquid media tested, Enriched Single Phase medium, which consists of Difco GC medium base (devoid of agar and starch) to which Lankford supplement and "Isovitalex" have been added, was the most suitable for use with small inocula.