TY - JOUR T1 - Can culture confirmation of gonococcal infection be improved in female subjects found to be positive by nucleic acid amplification tests in community clinics? JF - Sexually Transmitted Infections JO - Sex Transm Infect SP - 531 LP - 533 DO - 10.1136/sti.2009.036525 VL - 85 IS - 7 AU - G Gopal Rao AU - L Bacon AU - J Evans AU - Y Dejahang AU - R Hardwick AU - P Michalczyk AU - J Wong AU - A Donaldson Y1 - 2009/12/01 UR - http://sti.bmj.com/content/85/7/531.abstract N2 - Background: Use of nucleic acid amplification tests (NAATs), such as strand displacement assay (SDA, BD ProbeTec C trachomatis/N gonorrhoeae Amplified DNA Assay), for the detection of gonococcal infection in the community is controversial because of the possibility of false-positive results in low prevalence populations.Aim: To evaluate if culture confirmation of gonococcal infection can be improved for subjects found to be positive by BD ProbeTec in community clinics.Methods: Two cervical swabs were collected for culture to confirm NAAT positive results in women aged over 16 years—a majority of whom were <25 years and asymptomatic. One swab was urgently transported (UTP) and processed in the laboratory within 2 hours whereas the other swab (RTP) was stored at 4°C, transported at room temperature and processed 4–72 hours after collection depending on the time and day of collection.Results: Altogether, 56 subjects with NAAT positive results were recruited into the study. Nine (16.1%) subjects who were culture negative were excluded from final analysis due to prior antibiotic treatment (4/9) or the culture having been taken more than 1 month after the NAAT was positive (4/9) or an incorrect specimen being received (1/9). Overall, 41/47 (87.2%) NAAT positive subjects were confirmed by culture. In total, 40/47 (85.1%) UTP swabs and 27/47 (57.4%) RTP swabs were positive (p<0.05).Conclusion: This study shows that culture confirmation in NAAT positive subjects in a community gonococcus screening programme can be significantly improved by urgent transportation to and processing of specimens in the laboratory. ER -